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谷物培养的瘤胃真菌淀粉酶活性与蛋白酶活性的比较。

Comparison of amylolytic and proteolytic activities of ruminal fungi grown on cereal grains.

作者信息

Yanke L J, Dong Y, McAllister T A, Bae H D, Cheng K J

机构信息

Agriculture Canada Research Station, Lethbridge, AB.

出版信息

Can J Microbiol. 1993 Aug;39(8):817-20. doi: 10.1139/m93-121.

DOI:10.1139/m93-121
PMID:7693316
Abstract

Strains of the ruminal fungi Neocallimastix patriciarum, Orpinomyces joyonii, and Piromyces communis were grown on cellobiose and on cereal grains and then examined for proteolytic and amylolytic activities. On cellobiose all three fungi displayed similar activities, with the exception of little amylolytic activity in the cell-associated fraction of N. patriciarum. Growth on the cereal grains barley, corn, and wheat showed differences in proteolytic and amylolytic activities amongst the ruminal fungi and between the cereal grains. The data suggest that while these fungi are capable of fermenting the cereal grains the mode of enzymatic attack varies both with the particular fungus and the type of cereal grain.

摘要

瘤胃真菌梨形新美鞭菌(Neocallimastix patriciarum)、约氏奥尔平鞭菌(Orpinomyces joyonii)和普通梨囊鞭菌(Piromyces communis)的菌株在纤维二糖和谷物上培养,然后检测其蛋白水解和淀粉水解活性。在纤维二糖上,除了梨形新美鞭菌细胞相关部分几乎没有淀粉水解活性外,这三种真菌都表现出相似的活性。在大麦、玉米和小麦等谷物上生长时,瘤胃真菌之间以及谷物之间的蛋白水解和淀粉水解活性存在差异。数据表明,虽然这些真菌能够发酵谷物,但酶解攻击模式因特定真菌和谷物类型而异。

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