用于从呼吸道标本中检测卡氏肺孢子虫的单克隆抗体染色与荧光白染色的比较

Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens.

作者信息

Stratton N, Hryniewicki J, Aarnaes S L, Tan G, de la Maza L M, Peterson E M

机构信息

Department of Pathology, University of California, Irvine, Medical Center, Orange 92668.

出版信息

J Clin Microbiol. 1991 Mar;29(3):645-7. doi: 10.1128/jcm.29.3.645-647.1991.

DOI:10.1128/jcm.29.3.645-647.1991

PMID:1709951

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269837/

Abstract

Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expectorated sputum specimens. All stains detected the positive bronchoalveolar lavage specimens, the Genetic Systems stain detected six induced sputum specimens, and the other stains each detected four induced sputum specimens. The monoclonal antibody stains detected all three expectorated specimens, while the calcofluor stain detected only one. Overall, the sensitivities of the stains were 96% for Genetic Systems, 87% for both Disease Detection International and Meridian Diagnostics, and 78% for calcofluor.

摘要

对来自基因系统公司、国际疾病检测公司和子午线诊断公司的三种单克隆抗体染色试剂盒与荧光钙白进行了比较，以直接检测呼吸道标本中的卡氏肺孢子虫。在检测的150份标本中，四种染色方法中的任何一种都检测出23份标本卡氏肺孢子虫呈阳性；其中13份是支气管肺泡灌洗标本，7份是诱导痰标本，3份是咳出痰标本。所有染色方法都检测出了阳性支气管肺泡灌洗标本，基因系统公司的染色方法检测出6份诱导痰标本，其他染色方法各检测出4份诱导痰标本。单克隆抗体染色方法检测出了所有3份咳出痰标本，而荧光钙白染色方法仅检测出1份。总体而言，基因系统公司染色方法的灵敏度为96%，国际疾病检测公司和子午线诊断公司的染色方法均为87%，荧光钙白的灵敏度为78%。