Eremin A N, Metelitsa D I
Prikl Biokhim Mikrobiol. 1996 May-Jun;32(3):284-9.
Reversed micelles of Aerosol-OT (AOT) in heptane were used to purify superoxide dismutase (SOD) from bovine red blood cells. Effects of pH, temperature, concentrations of NaCl and protein in the aqueous phase, and the AOT to protein ratio on the extraction of the concomitant proteins from the aqueous into micellar phase were studied. The conditions were found that provided the extraction of concomitant proteins into micelles of AOT in heptane and left SOD in the aqueous phase. The SOD was electrophoretically pure; therefore, the need for its purification using chromatography was excluded.
使用正庚烷中气溶胶-OT（AOT）的反胶束从牛红细胞中纯化超氧化物歧化酶（SOD）。研究了pH值、温度、水相中NaCl和蛋白质的浓度以及AOT与蛋白质的比例对伴随蛋白质从水相萃取到胶束相的影响。发现了能将伴随蛋白质萃取到正庚烷中AOT胶束并使SOD留在水相中的条件。该SOD经电泳纯；因此，排除了使用色谱法对其进行纯化的必要性。
