Kersten C M, McCluskey R T, Shaw Warren H, Kurnick J T
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston.
Scand J Immunol. 1994 Aug;40(2):151-7. doi: 10.1111/j.1365-3083.1994.tb03444.x.
Normal human beings have circulating T lymphocytes that proliferate in response to Escherichia coli and Pseudomonas aeruginosa. We performed the present study to characterize the nature of the responding T cells and to determine whether distinct or shared conventional antigens, superantigens or polyclonal activators account for T cell proliferation. Long term antigen-specific T cell lines were generated by repeated stimulation of PBMC from four donors with soluble antigen preparations of E. coli or P. aeruginosa. This resulted in the emergence of distinct T cell populations, which responded to strains of either E. coli or P. aeruginosa, but not to both. Trypsin treatment of the bacterial preparations largely eliminated their ability to stimulate the T cells. The T cell lines were predominantly CD4+ and their proliferation to bacterial antigens was optimal using autologous APC. E. coli T cell lines proliferated not only in response to the E. coli strain with which they were initially selected, but also to four different strains of E. coli, as well as to several related Gram-negative species. P. aeruginosa selected T cells exhibited proliferative responses to six different P. aeruginosa strains, but not to the other Gram-negative species. The finding that repeated stimulation of PBMC with E. coli or P. aeruginosa leads to CD4+ T cells highly reactive with conventional protein antigens specific either for E. coli or P. aeruginosa indicates that these bacteria possess separate dominant protein antigens that drive the proliferation of peripheral blood T cells.
正常人体内循环的T淋巴细胞会对大肠杆菌和铜绿假单胞菌产生增殖反应。我们开展本研究以明确应答性T细胞的性质，并确定是不同的或共同的传统抗原、超抗原还是多克隆激活剂导致了T细胞增殖。通过用大肠杆菌或铜绿假单胞菌的可溶性抗原制剂反复刺激来自四名供体的外周血单核细胞（PBMC），生成了长期抗原特异性T细胞系。这导致出现了不同的T细胞群体，它们对大肠杆菌或铜绿假单胞菌的菌株有反应，但对两者都无反应。用胰蛋白酶处理细菌制剂在很大程度上消除了它们刺激T细胞的能力。T细胞系主要为CD4+，并且使用自体抗原呈递细胞（APC）时，它们对细菌抗原的增殖最为理想。大肠杆菌T细胞系不仅对最初用于筛选它们的大肠杆菌菌株有增殖反应，而且对四种不同的大肠杆菌菌株以及几种相关的革兰氏阴性菌也有反应。铜绿假单胞菌筛选出的T细胞对六种不同的铜绿假单胞菌菌株有增殖反应，但对其他革兰氏阴性菌无反应。用大肠杆菌或铜绿假单胞菌反复刺激PBMC会导致CD4+ T细胞对特定于大肠杆菌或铜绿假单胞菌的传统蛋白质抗原产生高度反应，这一发现表明这些细菌拥有各自独立的优势蛋白质抗原，可驱动外周血T细胞的增殖。
