Sciortino C V
Department of Microbiology, Veterans Administration Medical Center, Louisville, KY 40206.
Hybridoma. 1993 Jun;12(3):327-32. doi: 10.1089/hyb.1993.12.327.
We prepared an IgG1 murine monoclonal antibody, PS2, that recognized the 8-kDa outer membrane lipoprotein I of Pseudomonas aeruginosa. The biodot-immunoblot technique was used to test 470 bacterial isolates for their reactivity with MAb PS2. PS2 recognized 298 of 300 P. aeruginosa clinical isolates for a sensitivity of 99.3%. PS2 reacted with all Fisher-Devlin immunotype and International Antigenic Type P. aeruginosa strains. Forty-seven isolates of other Pseudomonas species were tested and only three P. cepacia reacted. PS2 did not react with 118 of 123 other gram-negative isolates, but reacted weakly with 3 of 17 Enterobacter aerogenes, 1 of 11 Eschericia coli and 1 of 2 Proteus vulgaris. The specificity of PS2 for P. aeruginosa was 95%.
我们制备了一种IgG1小鼠单克隆抗体PS2，它能识别铜绿假单胞菌8 kDa的外膜脂蛋白I。采用生物斑点免疫印迹技术检测了470株细菌分离株与单克隆抗体PS2的反应性。PS2识别出300株铜绿假单胞菌临床分离株中的298株，敏感性为99.3%。PS2与所有费舍尔-德夫林免疫型和国际抗原型铜绿假单胞菌菌株发生反应。对47株其他假单胞菌属菌株进行了检测，只有3株洋葱伯克霍尔德菌发生反应。PS2与123株其他革兰氏阴性菌分离株中的118株不发生反应，但与17株产气肠杆菌中的3株、11株大肠杆菌中的1株和2株普通变形杆菌中的1株发生弱反应。PS2对铜绿假单胞菌的特异性为95%。
