小鼠针对大肠杆菌和铜绿假单胞菌蛋白质抗原产生反应素抗体。

Reaginic antibody production to protein antigens of Escherichia coli and Pseudomonas aeruginosa by mice.

作者信息

Danneman P J, Michael J G

出版信息

Infect Immun. 1976 Sep;14(3):694-702. doi: 10.1128/iai.14.3.694-702.1976.

DOI:10.1128/iai.14.3.694-702.1976

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC420942/

Abstract

Water-soluble antigens isolated from acetone-dried, gram-negative bacteria elicited reaginic antibody formation in mice. Antibodies specific for Escherichia coli antigens reacted with antigens isolated from several enterobacterial species tested, but not with antigens isolated from Pseudomonas aeruginosa. Reaginic antibodies induced by antigens isolated from a P. aeruginosa strain reacted with antigens isolated from several P.aeruginosa serotypes as well as with a purified protein component of the envelope of P. aeruginosa. The anti-Pseudomonas reagins did not cross-react with enterobacterial antigens. Antigenicity of the bacterial extracts was destroyed by trypsin treatment and reduced by heating, which suggested that the antigens were protein in nature. Whole bacterial cells adsorbed out reaginic antibodies, indicating that the antigens are located at or near the surface of the bacteria.

摘要

从丙酮干燥的革兰氏阴性菌中分离出的水溶性抗原能在小鼠体内引发反应素抗体的形成。对大肠杆菌抗原具有特异性的抗体与从几种受试肠杆菌科细菌中分离出的抗原发生反应，但不与从铜绿假单胞菌中分离出的抗原发生反应。由铜绿假单胞菌菌株分离出的抗原诱导产生的反应素抗体与从几种铜绿假单胞菌血清型中分离出的抗原以及与铜绿假单胞菌包膜的一种纯化蛋白成分发生反应。抗铜绿假单胞菌反应素不与肠杆菌科抗原发生交叉反应。细菌提取物的抗原性经胰蛋白酶处理后被破坏，经加热后降低，这表明抗原本质上是蛋白质。完整的细菌细胞能吸附反应素抗体，这表明抗原位于细菌表面或其附近。

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