Adema G J, de Boer A J, Vogel A M, Loenen W A, Figdor C G
Division of Immunology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Amsterdam.
J Biol Chem. 1994 Aug 5;269(31):20126-33.
The glycoproteins recognized by monoclonal antibody (mAb) NKI-beteb are among the best diagnostic markers for human melanoma because their expression is restricted to melanocytic cells. Recently, we isolated a cDNA clone, termed gp100-c1, which confers immunoreactivity not only to mAb NKI-beteb, but also to two other mAbs used to diagnose malignant melanoma, HMB-50 and HMB-45. In this report, we demonstrate that gp100-c1 cDNA encodes glycoproteins of 100 kDa (gp100) and 10 kDa (gp10) which are recognized by these mAbs in human melanoma cells. The translation product deduced from the open reading frame present in gp100-c1 cDNA is highly homologous to another melanocyte-specific protein, Pmel17. Nucleotide sequence analysis of genomic DNA indicates that the transcripts corresponding to gp100 and Pmel17 cDNAs originate from a single gene via alternative splicing. In all normal and malignant melanocytic cells analyzed, gp100 and Pmel17 RNAs are simultaneously expressed.
单克隆抗体（mAb）NKI-beteb识别的糖蛋白是人类黑色素瘤最佳诊断标志物之一，因为它们的表达仅限于黑素细胞。最近，我们分离出一个名为gp100-c1的cDNA克隆，它不仅赋予对mAb NKI-beteb的免疫反应性，还赋予另外两种用于诊断恶性黑色素瘤的单克隆抗体HMB-50和HMB-45免疫反应性。在本报告中，我们证明gp100-c1 cDNA编码100 kDa（gp100）和10 kDa（gp10）的糖蛋白，它们在人类黑色素瘤细胞中被这些单克隆抗体识别。从gp100-c1 cDNA中的开放阅读框推导的翻译产物与另一种黑素细胞特异性蛋白Pmel17高度同源。基因组DNA的核苷酸序列分析表明，对应于gp100和Pmel17 cDNA的转录本通过可变剪接来自单个基因。在所有分析的正常和恶性黑素细胞中，gp100和Pmel17 RNA同时表达。
