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pMel17可被单克隆抗体NKI-beteb、HMB-45和HMB-50以及抗黑色素瘤细胞毒性T淋巴细胞识别。

pMel17 is recognised by monoclonal antibodies NKI-beteb, HMB-45 and HMB-50 and by anti-melanoma CTL.

作者信息

Adema G J, Bakker A B, de Boer A J, Hohenstein P, Figdor C G

机构信息

Department of Tumour Immunology, University Hospital Nijmegen St. Radboud, The Netherlands.

出版信息

Br J Cancer. 1996 May;73(9):1044-8. doi: 10.1038/bjc.1996.202.

DOI:10.1038/bjc.1996.202
PMID:8624261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2074403/
Abstract

Recently, we cloned the cDNA encoding the melanocyte lineage-specific antigen gp100 and demonstrated that gp100 is recognised by three different monoclonal antibodies (MAbs) used to diagnose malignant melanoma. In addition, we showed that tumour-infiltrating lymphocytes (TIL 1200) from a melanoma patient reacted specifically with cells transfected with the gp100 cDNA. Molecular characterisation of the gp100 cDNA revealed that the gp100 antigen is highly homologous, but not identical, to another melanocyte-specific protein, pMel17. Here, we report that cells transfected with pMel17 cDNA also react with all three MAbs used to diagnose malignant melanoma, NKI-beteb, HMB-45 and HMB-50. Moreover, pMel17 transfectants are specifically lysed by TIL1200. These data demonstrate that antigenic processing of both gp100 and pMel17 give rise to peptides seen by anti-melanoma cytotoxic T lymphocytes (CTL) and are therefore potential targets for immunotherapy of malignant melanoma.

摘要

最近，我们克隆了编码黑素细胞谱系特异性抗原gp100的cDNA，并证明gp100可被用于诊断恶性黑色素瘤的三种不同单克隆抗体（MAb）识别。此外，我们还表明，一名黑色素瘤患者的肿瘤浸润淋巴细胞（TIL 1200）与转染了gp100 cDNA的细胞发生特异性反应。gp100 cDNA的分子特征显示，gp100抗原与另一种黑素细胞特异性蛋白pMel17高度同源，但并不完全相同。在此，我们报告，转染了pMel17 cDNA的细胞也与用于诊断恶性黑色素瘤的所有三种MAb（NKI-beteb、HMB-45和HMB-50）发生反应。此外，pMel17转染子被TIL1200特异性裂解。这些数据表明，gp100和pMel17的抗原加工都会产生抗黑色素瘤细胞毒性T淋巴细胞（CTL）可见的肽段，因此是恶性黑色素瘤免疫治疗的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/2074403/fe3326859133/brjcancer00037-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/2074403/f05aa5e4751a/brjcancer00037-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/2074403/fe3326859133/brjcancer00037-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/2074403/f05aa5e4751a/brjcancer00037-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/2074403/fe3326859133/brjcancer00037-0026-a.jpg

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本文引用的文献

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Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes.
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Sci Rep. 2012;2:573. doi: 10.1038/srep00573. Epub 2012 Aug 13.
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The secreted form of a melanocyte membrane-bound glycoprotein (Pmel17/gp100) is released by ectodomain shedding.黑素细胞膜结合糖蛋白（Pmel17/gp100）的分泌形式通过细胞外结构域脱落释放。
FASEB J. 2010 Mar;24(3):916-30. doi: 10.1096/fj.09-140921. Epub 2009 Nov 2.
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A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats.一种由人类黑素细胞和黑色素瘤细胞表达的新型Pmel17剪接变体，其缺少一些内部重复序列。
J Invest Dermatol. 2003 Oct;121(4):821-30. doi: 10.1046/j.1523-1747.2003.12474.x.
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