• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Isolation of DNA fragments from agarose gel by centrifugation.

作者信息

Wang Z, Rossman T G

机构信息

Nelson Institute of Environmental Medicine, NYU Medical Center, NY 10016.

出版信息

Nucleic Acids Res. 1994 Jul 25;22(14):2862-3. doi: 10.1093/nar/22.14.2862.

DOI:10.1093/nar/22.14.2862
PMID:7519772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308261/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c4/308261/19f91457c2f0/nar00038-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c4/308261/155ff856ba31/nar00038-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c4/308261/19f91457c2f0/nar00038-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c4/308261/155ff856ba31/nar00038-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c4/308261/19f91457c2f0/nar00038-0155-a.jpg

相似文献

1
Isolation of DNA fragments from agarose gel by centrifugation.通过离心从琼脂糖凝胶中分离DNA片段。
Nucleic Acids Res. 1994 Jul 25;22(14):2862-3. doi: 10.1093/nar/22.14.2862.
2
Capillary electrophoresis of DNA fragments with replaceable low-gelling agarose gels.使用可替换的低熔点琼脂糖凝胶对DNA片段进行毛细管电泳。
Methods Mol Biol. 2001;162:279-90. doi: 10.1385/1-59259-055-1:279.
3
Southern blotting of agarose gels by capillary transfer.通过毛细管转移对琼脂糖凝胶进行Southern印迹法。
Methods Mol Biol. 2002;187:23-7. doi: 10.1385/1-59259-273-2:023.
4
Elution of DNA from agarose gels after electrophoresis.电泳后从琼脂糖凝胶中洗脱DNA。
Methods Enzymol. 1979;68:176-82. doi: 10.1016/0076-6879(79)68012-6.
5
The resolution of DNA fragments in capillary electrophoresis in replaceable agarose gels.可替换琼脂糖凝胶中毛细管电泳法对DNA片段的分离
J Capillary Electrophor. 1996 May-Jun;3(3):173-9.
6
Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis).通过置换电泳（等速电泳）从凝胶中快速定量回收DNA片段。
Biochim Biophys Acta. 1984 Jun 16;782(2):120-6. doi: 10.1016/0167-4781(84)90014-9.
7
Optimized centrifugation for rapid elution of DNA from agarose gels.
Genet Anal Tech Appl. 1992 Feb;9(1):31-3. doi: 10.1016/1050-3862(92)90027-3.
8
A simple and efficient method for the separation and detection of small DNA fragments by electrophoresis in formamide containing agarose gels and Southern blotting to DBM-paper.一种通过在含甲酰胺的琼脂糖凝胶中进行电泳以及对DBM纸进行Southern印迹来分离和检测小DNA片段的简单高效方法。
Nucleic Acids Res. 1982 Oct 11;10(19):5753-63. doi: 10.1093/nar/10.19.5753.
9
Agarose Gel Size Selection for DNA Sequencing Libraries.
Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.prot094698. doi: 10.1101/pdb.prot094698.
10
Rapid DNA elution procedure from agarose gels.
Anal Biochem. 1996 Mar 1;235(1):106-7. doi: 10.1006/abio.1996.0099.

引用本文的文献

1
An affordable and simple method for DNA extraction from agarose suitable for downstream applications.一种经济实惠且简单的从琼脂糖中提取DNA的方法，适用于下游应用。
Sci Rep. 2025 Mar 26;15(1):10414. doi: 10.1038/s41598-025-87572-w.
2
Induction of Monoterpenoid Oxindole Alkaloids Production and Related Biosynthetic Gene Expression in Response to Signaling Molecules in Plant Cultures.植物培养物中响应信号分子诱导单萜吲哚生物碱的产生及相关生物合成基因的表达
Plants (Basel). 2024 Mar 27;13(7):966. doi: 10.3390/plants13070966.
3
A reassessment of several erstwhile methods for isolating DNA fragments from agarose gels.

本文引用的文献

1
dbEST--database for "expressed sequence tags".dbEST——“表达序列标签”数据库。
Nat Genet. 1993 Aug;4(4):332-3. doi: 10.1038/ng0893-332.
2
GenBank.基因银行
Nucleic Acids Res. 1993 Jul 1;21(13):2963-5. doi: 10.1093/nar/21.13.2963.
3
Issues in searching molecular sequence databases.搜索分子序列数据库中的问题。
对几种曾用于从琼脂糖凝胶中分离DNA片段的方法的重新评估。
3 Biotech. 2021 Mar;11(3):138. doi: 10.1007/s13205-021-02691-1. Epub 2021 Feb 23.
4
DNA polymerase ζ-dependent lesion bypass in Saccharomyces cerevisiae is accompanied by error-prone copying of long stretches of adjacent DNA.酿酒酵母中依赖DNA聚合酶ζ的损伤旁路伴随着相邻DNA长片段的易错复制。
PLoS Genet. 2015 Mar 31;11(3):e1005110. doi: 10.1371/journal.pgen.1005110. eCollection 2015 Mar.
5
Large scale isolation of expression vector cassette by magnetic triple helix affinity capture.通过磁性三螺旋亲和捕获大规模分离表达载体盒。
Nucleic Acids Res. 1995 Oct 11;23(19):3995-6. doi: 10.1093/nar/23.19.3995.
Nat Genet. 1994 Feb;6(2):119-29. doi: 10.1038/ng0294-119.
4
A common language for physical mapping of the human genome.人类基因组物理图谱的通用语言。
Science. 1989 Sep 29;245(4925):1434-5. doi: 10.1126/science.2781285.
5
Extraction of nucleic acids from agarose gel--a quantitative and qualitative comparison of four different methods.从琼脂糖凝胶中提取核酸——四种不同方法的定量和定性比较
Prep Biochem. 1990;20(2):123-35. doi: 10.1080/00327489008050184.
6
Complementary DNA sequencing: expressed sequence tags and human genome project.互补DNA测序：表达序列标签与人类基因组计划
Science. 1991 Jun 21;252(5013):1651-6. doi: 10.1126/science.2047873.