Morino P, Mascagni F, McDonald A, Hökfelt T
Department of Neuroscience and Anatomy, Karolinska Institut, Stockholm, Sweden.
Neuroscience. 1994 Apr;59(4):939-52. doi: 10.1016/0306-4522(94)90297-6.
The anterograde tracer Phaseolus vulgaris-leucoagglutinin was used to examine the organization of the projections to the striatum from medial prefrontal and frontal cortical areas in the rat with reference to their relation to cholecystokinin-like immunoreactivity in the striatum. Medial prefrontal cortical areas projected bilaterally, with an ipsilateral predominance, to the striatum. Most of the positive fibres were found in medial and ventral areas of the caudate-putamen and in the nucleus accumbens. Labelled fibres formed distinct patch-like arrangements throughout the dorsomedial striatum, whereas more ventrally the fibres were densely packed and spread to lateral areas. Almost no fibres were found in the dorsolateral aspects of the caudate-putamen. Cholecystokinin-like immunoreactivity in the striatum was diffusely distributed in the medial aspects, in fine punctate elements as well as in patches of fibres. Overlapping of corticostriatal clusters of fibres, from medial prefrontal cortex, with cholecystokinin-immunoreactive patches was found at all rostrocaudal levels studied, but predominantly in rostral areas. The overlap was present both in the ipsilateral and the contralateral side. Often the cluster of corticostriatal fibres was completely and precisely overlaid by a cholecystokinin-immunoreactive patch. At more caudal planes the overlap was only partial and in some instances cholecystokinin-positive patches "avoided" zones of dense corticostriatal fibre terminations. Frontal cortex injections of tracer gave rise to a network of fibres in the lateral aspects of the striatum, sparing the medial areas. No overlap with cholecystokinin-immunoreactive patches was found in these cases. These results suggest that a large number of cholecystokinin-containing striatal fibres originate in medial prefrontal cortical areas.
运用顺行示踪剂菜豆白细胞凝集素，参照其与纹状体中胆囊收缩素样免疫反应性的关系，研究大鼠内侧前额叶和额叶皮质区域向纹状体投射的组织情况。内侧前额叶皮质区域双侧投射至纹状体，以同侧为主。大多数阳性纤维见于尾壳核的内侧和腹侧区域以及伏隔核。标记纤维在背内侧纹状体中形成明显的斑块样排列，而在更腹侧区域，纤维密集排列并延伸至外侧区域。在尾壳核的背外侧部分几乎未发现纤维。纹状体中的胆囊收缩素样免疫反应性在内侧区域呈弥漫性分布，存在于细小的点状成分以及纤维斑块中。在所研究的所有 rostrocaudal 水平上，均发现来自内侧前额叶皮质的皮质纹状体纤维簇与胆囊收缩素免疫反应性斑块存在重叠，但主要见于 Rostral 区域。同侧和对侧均存在重叠。皮质纹状体纤维簇常常完全且精确地被胆囊收缩素免疫反应性斑块覆盖。在更靠尾侧的层面，重叠仅为部分，在某些情况下，胆囊收缩素阳性斑块“避开”了皮质纹状体纤维密集终末的区域。向额叶皮质注射示踪剂后，在纹状体外侧形成纤维网络，内侧区域未受影响。在这些情况下，未发现与胆囊收缩素免疫反应性斑块的重叠。这些结果表明，大量含胆囊收缩素的纹状体纤维起源于内侧前额叶皮质区域。
