Arnalich F, de Miguel E, Perez-Ayala C, Martinez M, Vazquez J J, Gijon-Banos J, Hernanz A
Department of Medicine, Autonomous University, Madrid, Spain.
Neurosci Lett. 1994 Apr 11;170(2):251-4. doi: 10.1016/0304-3940(94)90331-x.
Plasma and synovial fluid concentrations of interleukin-6 (IL-6), using an enzyme-linked immunosorbent assay, as well as immunoreactive levels of calcitonin gene-related peptide (CGRP), substance P and vasoactive intestinal peptide (VIP) were measured in 18 patients with rheumatoid arthritis and 20 with osteoarthritis of the knee. The concentrations of IL-6 were elevated in both plasma and synovial fluids from patients with rheumatoid arthritis whereas higher levels of substance P-, CGRP- and VIP-like immunoreactivities were found in the synovial fluid, but not in plasma, from patients with rheumatoid arthritis when compared with those in osteoarthritis. Furthermore, IL-6 and substance P levels in synovial fluid were significantly correlated both in rheumatoid arthritis and osteoarthritis patients. Our data seem to support the idea of an important role shared by neuropeptides and IL-6 in the pathogenesis of human inflammatory joint disease.
采用酶联免疫吸附测定法，对18例类风湿关节炎患者和20例膝骨关节炎患者的血浆和滑液中白细胞介素-6（IL-6）浓度，以及降钙素基因相关肽（CGRP）、P物质和血管活性肠肽（VIP）的免疫反应水平进行了测定。类风湿关节炎患者的血浆和滑液中IL-6浓度均升高，而与骨关节炎患者相比，类风湿关节炎患者滑液中P物质、CGRP和VIP样免疫反应性水平较高，但血浆中未出现这种情况。此外，类风湿关节炎和骨关节炎患者滑液中的IL-6和P物质水平均显著相关。我们的数据似乎支持神经肽和IL-6在人类炎性关节疾病发病机制中共同发挥重要作用这一观点。
