Larsson J, Ekblom A, Henriksson K, Lundeberg T, Theodorsson E
Department of Physiology II, Karolinska Institute, Stockholm, Sweden.
Scand J Rheumatol. 1991;20(5):326-35. doi: 10.3109/03009749109096808.
We have studied the presence of five neuropeptides in knee joint synovial fluid from either patients suffering from rheumatoid arthritis and pain (n = 18) or being subjected to arthroscopy due to meniscal/cruciate ligament injuries (n = 13). Radioimmunoassay technique was used for peptide analysis using antisera SP2 against substance P (SP), K12 against neurokinin A (NKA), CGRPR8 against calcitonin gene-related peptide (CGRP), NPY1 against neuropeptide Y (NPY) and VIP2 against vasoactive intestinal polypeptide (VIP). No SP could be detected, and lower levels of NKA was found in arthritic joints vs controls. CGRP and NPY was found in higher concentrations in arthritic patients vs controls. VIP was found sporadically in both arthritis and control patients. Our data show some quantitative differences between patients suffering rheumatoid arthritis and pain, and patients with non-inflamed joints without pain; indicating an involvement of peptidergic fibers in arthritis in humans.
我们研究了类风湿性关节炎伴疼痛患者（n = 18）或因半月板/十字韧带损伤接受关节镜检查患者（n = 13）膝关节滑液中五种神经肽的存在情况。采用放射免疫测定技术，使用针对P物质（SP）的抗血清SP2、针对神经激肽A（NKA）的K12、针对降钙素基因相关肽（CGRP）的CGRPR8、针对神经肽Y（NPY）的NPY1和针对血管活性肠肽（VIP）的VIP2进行肽分析。未检测到SP，且与对照组相比，关节炎关节中NKA水平较低。与对照组相比，关节炎患者中CGRP和NPY浓度较高。VIP在关节炎患者和对照患者中均偶尔发现。我们的数据显示，类风湿性关节炎伴疼痛患者与无疼痛的非炎症关节患者之间存在一些定量差异；表明肽能纤维参与了人类关节炎的发病过程。
