Wyss P, Tadir Y, Tromberg B J, Liaw L, Krasieva T, Berns M W
Beckman Laser Institute and Medical Clinic, University of California, Irvine.
Obstet Gynecol. 1994 Sep;84(3):409-14.
To determine the optimal pharmacokinetic characteristics for photodynamic endometrial destruction using topically applied benzoporphyrin derivative and to evaluate long-term morphologic effects in a rabbit model.
Benzoporphyrin derivative pharmacokinetics were measured by analyzing frozen tissue sections 1.5-12 hours following topical administration. Photodynamic therapy was induced intraluminally 1.5 hours after drug application, and tissue morphology was evaluated by light and scanning electron microscopy 1 and 4 weeks after treatment.
The highest glandular and stromal fluorescence was observed 1.5 hours after topical administration. Relative fluorescence of the endometrial glands significantly exceeded that of stroma and myometrium mainly at 1.5 and 3 hours (P < .0001). Histology revealed persistent epithelial destruction with minimal regeneration. Stroma resembled scar tissue in the majority of sections. The bordering myometrium was loosened and invaded by connective tissue.
Topically applied benzoporphyrin derivative can be used for highly effective, long-lasting photodynamic destruction of rabbit endometrium. However, optical dosimetry can vary, particularly in the rabbit model, and this appears to affect long-term reepithelialization.
确定使用局部应用的苯并卟啉衍生物进行光动力子宫内膜破坏的最佳药代动力学特征，并评估兔模型中的长期形态学效应。
在局部给药后1.5至12小时，通过分析冷冻组织切片来测量苯并卟啉衍生物的药代动力学。在给药后1.5小时腔内诱导光动力疗法，并在治疗后1周和4周通过光学显微镜和扫描电子显微镜评估组织形态。
局部给药后1.5小时观察到最高的腺和基质荧光。子宫内膜腺体的相对荧光主要在1.5小时和3小时时显著超过基质和肌层（P <.0001）。组织学显示持续的上皮破坏且再生极少。在大多数切片中，基质类似瘢痕组织。毗邻的肌层疏松并被结缔组织侵入。
局部应用苯并卟啉衍生物可用于兔子宫内膜的高效、持久光动力破坏。然而，光剂量测定可能会有所不同，特别是在兔模型中，这似乎会影响长期再上皮化。
