5-氨基酮戊酸诱导的光动力疗法后大鼠子宫内膜的光敏化作用

Photosensitization of the rat endometrium following 5-aminolevulinic acid induced photodynamic therapy.

作者信息

Steiner R A, Tadir Y, Tromberg B J, Krasieva T, Ghazains A T, Wyss P, Berns M W

机构信息

Beckman Laser Institute and Medical Clinic, University of California, Irvine 92715, USA.

出版信息

Lasers Surg Med. 1996;18(3):301-8. doi: 10.1002/(SICI)1096-9101(1996)18:3<301::AID-LSM12>3.0.CO;2-8.

DOI:10.1002/(SICI)1096-9101(1996)18:3<301::AID-LSM12>3.0.CO;2-8

PMID:8778526

Abstract

BACKGROUND AND OBJECTIVE

The impact of photodynamic therapy (PDT) on the endometrium following topical application of 5-aminolevulinic acid (ALA) was studied in a rat model. Study Design/

MATERIALS AND METHODS

Fluorescence microscopy revealed peak ALA to protoporphyrin IX (Pp IX) conversion 3-6 hours after application. Significantly higher Pp IX levels were observed in the endometrial glands compared with endometrial stroma and myometrium.

RESULTS

Histological studies showed PDT-induced endometrial destruction with atrophy 7-10 weeks after treatment. Reproductive performance studies demonstrated significant implantation failure in the treated uterine horns compared with controls. The number of implantation sacs in the treated and untreated horns was 0.4 +/- 0.3 and 8.9 +/- 1.0, respectively (P < 0.01).

CONCLUSION

We conclude that the high rate of Pp IX conversion in the endometrial glands can be exploited to treat dysfunctional uterine bleeding with PDT. In addition, this concept may also be applied to study endometrial regeneration and embryo implantation mechanisms.

摘要

背景与目的

在大鼠模型中研究了局部应用5-氨基乙酰丙酸（ALA）后光动力疗法（PDT）对子宫内膜的影响。研究设计/材料与方法：荧光显微镜显示，应用ALA后3 - 6小时，ALA向原卟啉IX（Pp IX）的转化达到峰值。与子宫内膜基质和肌层相比，在子宫内膜腺体中观察到显著更高的Pp IX水平。结果：组织学研究显示，治疗后7 - 10周，PDT诱导子宫内膜破坏并萎缩。生殖性能研究表明，与对照组相比，治疗侧子宫角的着床失败显著。治疗侧和未治疗侧子宫角的着床囊数量分别为0.4±0.3和8.9±1.0（P<0.01）。结论：我们得出结论，子宫内膜腺体中Pp IX的高转化率可用于通过PDT治疗功能失调性子宫出血。此外，这一概念也可应用于研究子宫内膜再生和胚胎着床机制。