Stuyver L, van Arnhem W, Wyseur A, Maertens G
Innogenetics N. V., Gent, Belgium.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1308-14. doi: 10.1006/bbrc.1994.2073.
By means of the Line Probe Assay, a hepatitis C virus type 5a infected serum from a Belgian patient was selected. The complete core region (573 bp), the carboxyterminal part of E1 and the aminoterminal part of E2/NS1 (661 bp), and an epitope containing region in NS3-NS4 (1452 bp) were cloned and sequenced. The deduced amino acid sequence revealed type-specific variations in regions of core and NS4 which were previously recognized as evoking a type-specific antibody response. In addition, the aminoterminal region of E2 showed high variability when compared with sequences of type 1, 2, and 3. Phylogenetic analysis showed separate branching of this isolate. The analysis in the core region was not conclusive for some of the strains included and should therefore be carried out in combination with the NS5B region.
通过线性探针分析，从一名比利时患者的血清中筛选出了感染5a型丙型肝炎病毒的样本。对完整的核心区域（573bp）、E1的羧基末端部分和E2/NS1的氨基末端部分（661bp）以及NS3-NS4中的一个表位包含区域（1452bp）进行了克隆和测序。推导的氨基酸序列显示，核心区域和NS4区域存在类型特异性变异，这些区域先前被认为会引发类型特异性抗体反应。此外，与1型、2型和3型序列相比，E2的氨基末端区域显示出高度变异性。系统发育分析表明该分离株有单独的分支。对于所纳入的一些毒株，核心区域的分析结果并不确定，因此应结合NS5B区域进行分析。
