Stuyver L, Van Arnhem W, Wyseur A, DeLeys R, Maertens G
Innogenetics N.V., Gent, Belgium.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):635-41. doi: 10.1006/bbrc.1993.1462.
Using a Line Probe Assay, type 3 HCV genotype-infected sera were selected from Brazilian blood donors. The partial nucleotide sequences of the core/E1 and NS4a epitope-containing regions and the NS5b typing region were determined. The E1 region had a nucleic acid homology of only 61 to 65% with the type 1 prototype genomes, and 56 to 58% homology with the type 2 prototype HCV genomes. Similar homologies were also found for the NS4a epitope region and for NS5. Furthermore, the deduced amino acid sequence of type 3 NS4a was used to generate synthetic peptides which were strongly reactive with human HCV-infected sera which were previously determined as anti-NS4 negative, indicating that a type-specific antibody response to the NS4a protein may exist.
使用线性探针分析，从巴西献血者中筛选出感染3型丙型肝炎病毒（HCV）基因型的血清。测定了核心/E1和含NS4a表位区域以及NS5b分型区域的部分核苷酸序列。E1区域与1型原型基因组的核酸同源性仅为61%至65%，与2型原型HCV基因组的同源性为56%至58%。在NS4a表位区域和NS5区域也发现了类似的同源性。此外，3型NS4a的推导氨基酸序列被用于生成合成肽，这些合成肽与先前被确定为抗NS4阴性的人类HCV感染血清强烈反应，表明可能存在针对NS4a蛋白的型特异性抗体反应。
