Lutomski D, Caron M, Bourin P, Lefebure C, Bladier D, Joubert-Caron R
Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, UFR SMBH Léonard de Vinci, Bobigny, France.
J Neuroimmunol. 1995 Mar;57(1-2):9-15. doi: 10.1016/0165-5728(94)00152-e.
We have recently identified oligoclonal IgG antibodies that are related to a human brain lectin (HBL14) from serum and cerebrospinal fluid of patients with neurological disorders. They were termed lectin-like IgG (L-IgG) (Joubert-Caron et al., 1994a,b). In this paper, the occurrence of antibodies reactive both towards HBL14 and L-IgG was investigated. Binding of antibodies to HBL14 was demonstrated by solid-phase ELISA and chromatography on immobilized HBL14. Fab fragments of these antibodies were also shown to bind to HBL14. The specificity of the antibodies towards HBL14 was studied using a panel of different antigens. Our data show that individual sera from healthy people as well as a pool of immunoglobulins from 80 blood donors contain an IgG autoreactivity to HBL14, while no IgM autoreactivity was detected. Anti-HBL14 antibodies from sera were purified using affinity chromatography on immobilized HBL14. Affinity chromatography further allowed us to demonstrate that the binding of anti-HB14 antibodies was mediated through their Fab fragments. A higher amount of anti-HBL14 antibodies was purified using a L-IgG-depleted fraction of sera. The binding of anti-HBL14 antibodies to L-IgG was confirmed by ELISA. Finally, anti-HBL14 antibodies were found to be polyreactive. These results indicate the occurrence of a novel class of natural antibodies reactive towards a human brain lectin and suggest that these antibodies may participate in immunoregulatory mechanisms probably though idiotypic/anti-idiotypic interaction.
我们最近从神经疾病患者的血清和脑脊液中鉴定出了与一种人脑凝集素（HBL14）相关的寡克隆IgG抗体。它们被称为凝集素样IgG（L-IgG）（朱伯特 - 卡龙等人，1994a，b）。在本文中，研究了对HBL14和L-IgG均有反应的抗体的出现情况。通过固相ELISA和固定化HBL14上的色谱法证明了抗体与HBL14的结合。这些抗体的Fab片段也显示与HBL14结合。使用一组不同的抗原研究了抗体对HBL14的特异性。我们的数据表明，健康人的个体血清以及80名献血者的免疫球蛋白池中都含有对HBL14的IgG自身反应性，而未检测到IgM自身反应性。使用固定化HBL14上的亲和色谱法从血清中纯化抗HBL14抗体。亲和色谱法进一步使我们能够证明抗HB14抗体的结合是通过其Fab片段介导的。使用血清的L-IgG缺失部分纯化出了更高量的抗HBL14抗体。通过ELISA证实了抗HBL14抗体与L-IgG的结合。最后，发现抗HBL14抗体具有多反应性。这些结果表明存在一类对人脑凝集素具有反应性的新型天然抗体，并表明这些抗体可能通过独特型/抗独特型相互作用参与免疫调节机制。
