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A simplified method for single-cell RT-PCR that can detect and distinguish genomic DNA and mRNA transcripts.

作者信息

Tong J, Bendahhou S, Chen H, Agnew W S

机构信息

Department of Physiology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205.

出版信息

Nucleic Acids Res. 1994 Aug 11;22(15):3253-4. doi: 10.1093/nar/22.15.3253.

DOI:10.1093/nar/22.15.3253
PMID:7520571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310308/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/310308/6a95fce1a000/nar00039-0399-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/310308/6a95fce1a000/nar00039-0399-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4642/310308/6a95fce1a000/nar00039-0399-a.jpg

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EMBO J. 1993 Dec 15;12(13):4955-67. doi: 10.1002/j.1460-2075.1993.tb06189.x.
2
A single B1 subunit mapped to mouse chromosome 7 may be a common component of Na channel isoforms from brain, skeletal muscle and heart.定位到小鼠7号染色体的单个B1亚基可能是来自脑、骨骼肌和心脏的钠通道亚型的共同组成部分。
Biochem Biophys Res Commun. 1993 Sep 15;195(2):679-85. doi: 10.1006/bbrc.1993.2099.
3
Co-amplification of the cystic fibrosis delta F508 mutation with the HLA DQA1 sequence in single cell PCR: implications for improved assessment of polar bodies and blastomeres in preimplantation diagnosis.
热处理后通过逆转录聚合酶链反应直接检测受污染牛奶样品中的活菌、霉菌和酵母菌。
Appl Environ Microbiol. 1998 Mar;64(3):1157-60. doi: 10.1128/AEM.64.3.1157-1160.1998.
4
Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase.利用核苷酸类似物dITP和嗜热栖热菌DNA聚合酶对RNA进行选择性扩增。
Nucleic Acids Res. 1996 Dec 15;24(24):5021-5. doi: 10.1093/nar/24.24.5021.
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Single-cell analysis of gene expression in the nervous system. Measurements at the edge of chaos.
Mol Neurobiol. 1996 Dec;13(3):199-211. doi: 10.1007/BF02740623.
6
Serine-1321-independent regulation of the mu 1 adult skeletal muscle Na+ channel by protein kinase C.蛋白激酶C对μ1成年骨骼肌钠通道的丝氨酸1321非依赖性调节。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12003-7. doi: 10.1073/pnas.92.26.12003.
单细胞聚合酶链反应中囊性纤维化ΔF508突变与HLA DQA1序列的共扩增：对植入前诊断中极体和卵裂球评估改进的意义
Prenat Diagn. 1993 Dec;13(12):1111-22. doi: 10.1002/pd.1970131206.
4
Subunit composition at the single-cell level explains functional properties of a glutamate-gated channel.单细胞水平的亚基组成解释了谷氨酸门控通道的功能特性。
Neuron. 1994 Feb;12(2):383-8. doi: 10.1016/0896-6273(94)90279-8.
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Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
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Construction of cDNA libraries from small numbers of cells using sequence independent primers.使用序列非依赖性引物从少量细胞构建cDNA文库。
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Primary structure and functional expression of a mammalian skeletal muscle sodium channel.哺乳动物骨骼肌钠通道的一级结构与功能表达
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Novel method for studying mRNA phenotypes in single or small numbers of cells.研究单个或少量细胞中mRNA表型的新方法。
J Cell Biochem. 1989 Jan;39(1):1-11. doi: 10.1002/jcb.240390102.