Yamaguchi T, Tanaka S, Shigeta S, Wada T, Tsuboi S, Otsuka T, Katsutani T, Jyo T, Oka S, Ono K
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University.
Arerugi. 1994 Jun;43(6):701-10.
A significant activity to elicit histamine release was found in an ultrafilterable (Mr-cutoff < 10 kD) fraction of a mite feces extract from a spent mite medium. The activity was divided into two fractions (LM1 and LM2) on an Ultrogel AcA 54 column when monitored by histamine release assay using washed peripheral blood cells from mite-allergic patients. The larger-molecular-size antigen, LM1 was further separated into three allergenic fractions by consecutive chromatography on Sephadex G-50, GM-DEAE, and TSKgel ODS-120T. All the purified allergens, LM1s (LM1a, LM1b, and LM1c), which produced positive reactions in skin tests on allergic patients, were glycoproteins (molecular weight: 8 kD on SDS-PAGE; 12.5 kD on Gel filtration) with different carbohydrate contents and pI values ranging from 4 to 5 on an IEF plate. LM1s were cross-reactive with anti-Der f II but not with anti-Der f I. The reaction of LM1c to anti-Der f II serum was completely inhibited with the other antigens, while the reactions of LM1a and LM1b to the serum were partially inhibited with LM1c or the other antigens, respectively.
在来自用过的螨虫培养基的螨虫粪便提取物的超滤部分（截留分子量<10 kD）中发现了一种显著的引发组胺释放的活性物质。当使用螨虫过敏患者的洗涤外周血细胞通过组胺释放试验进行监测时，该活性物质在Ultrogel AcA 54柱上被分离成两个部分（LM1和LM2）。分子量较大的抗原LM1通过在Sephadex G - 50、GM - DEAE和TSKgel ODS - 120T上连续色谱进一步分离成三个过敏原部分。所有在过敏患者皮肤试验中产生阳性反应的纯化过敏原LM1s（LM1a、LM1b和LM1c）都是糖蛋白（SDS - PAGE上分子量为8 kD；凝胶过滤上为12.5 kD），具有不同的碳水化合物含量，在IEF平板上的pI值范围为4至5。LM1s与抗Der f II交叉反应，但不与抗Der f I交叉反应。LM1c与抗Der f II血清的反应被其他抗原完全抑制，而LM1a和LM1b与该血清的反应分别被LM1c或其他抗原部分抑制。
