Yamaguchi T, Tanaka S, Shigeta S, Wada T, Tsuboi S, Otsuka T, Katsutani T, Jyo T, Oka S, Ono K
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University.
Arerugi. 1994 Jul;43(7):815-24.
A low-molecular-size antigen, LM2, which possesses a significant activity to elicit histamine release, was isolated from an ultrafilterable (Mr-cutoff < 10k) fraction of a mite extract containing feces by consecutive chromatography on Ultrogel AcA 54 and Sephadex G-25. The isolated antigen was still heterogeneous glycoproteins, giving a smeary band around pI 3-5 on an Ampholine PAG plate, with molecular weights of 6-8k and 4k on SDS-PAGE and Sephadex G-50 gel filtration, respectively. Chemical treatments of the antigen suggested that the epitope responsible for the elicitation of histamine release resided in the protein moiety. The antigen had activities to provoke conjunctival congestion and histamine release in mite-allergic patients, but not immunogenicity by itself. The antigen competitively inhibited the reactions of HM1, HM2, and HM3 fractions to corresponding antisera, but did not cross-react with anti-Der f I or anti-Der f II sera.
从含有粪便的螨提取物的超滤部分（截留分子量<10k）中，通过在Ultrogel AcA 54和Sephadex G-25上连续层析，分离出一种具有显著组胺释放活性的低分子大小抗原LM2。分离出的抗原仍然是异质性糖蛋白，在两性电解质聚丙烯酰胺凝胶板上，在等电点3 - 5附近呈现弥散条带，在十二烷基硫酸钠聚丙烯酰胺凝胶电泳和Sephadex G-50凝胶过滤中，分子量分别为6 - 8k和4k。对抗原的化学处理表明，负责引发组胺释放的表位存在于蛋白质部分。该抗原能引起螨过敏患者的结膜充血和组胺释放，但本身无免疫原性。该抗原竞争性抑制HM1、HM2和HM3部分与相应抗血清的反应，但不与抗Der f I或抗Der f II血清发生交叉反应。
