Nakazawa T, Kato N, Ohkoshi S, Shibuya A, Shimotohno K
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
J Hepatol. 1994 May;20(5):623-9. doi: 10.1016/s0168-8278(05)80350-2.
We examined 14 patients with hepatitis C caused by infection with the hepatitis C virus-II genotype to understand differences in responsiveness to interferon. The patients were classified into two groups according to their response to interferon: eight responding and six non-responding patients. The 5' noncoding and structural regions of the hepatitis C virus-II genome from each patient specimen were amplified by reverse transcription followed by the polymerase chain reaction. The nucleotide sequences of these amplified DNAs were then determined. By comparing the nucleotide sequences and the deduced amino acid sequences of samples from both groups, no group-specific sequence was observed in the analyzed regions despite the presence of considerable sequence diversity. However, additional cysteine residues were observed in half the responding group. The degree of micro-heterogeneity in hypervariable region 1 of the hepatitis C virus in relation to the sensitivity to interferon treatment was also examined; however, no significant correlation was observed. In addition, frequent alterations in the amino acid sequences were observed in hypervariable region 1 during the course of interferon treatment.
我们检查了14例由丙型肝炎病毒II基因型感染引起的丙型肝炎患者，以了解对干扰素反应的差异。根据患者对干扰素的反应将其分为两组：8例有反应患者和6例无反应患者。通过逆转录随后进行聚合酶链反应，扩增每个患者标本中丙型肝炎病毒II基因组的5'非编码区和结构区。然后确定这些扩增DNA的核苷酸序列。通过比较两组样本的核苷酸序列和推导的氨基酸序列，尽管存在相当大的序列多样性，但在所分析的区域中未观察到组特异性序列。然而，在一半的有反应组中观察到了额外的半胱氨酸残基。还检查了丙型肝炎病毒高变区1中微异质性程度与干扰素治疗敏感性的关系；然而，未观察到显著相关性。此外，在干扰素治疗过程中，高变区1的氨基酸序列频繁发生改变。
