Chayama K
Department of Gastroenterology, Toranomon Hospital.
Nihon Rinsho. 1994 Jul;52(7):1716-20.
The hypervariable region 1 of the hepatitis C virus genome has attracted much scientific interest as higher degrees of variability of the region have been reported to be a predictive factor of poor response to interferon treatment. We analyzed sequential changes in the amino acid sequence of this region before, during and after two courses of interferon treatment. A major strain seen before treatment was overtaken by another strain which had been a relatively minor population before therapy. Later a transient emergence of two minor populations was observed during interferon treatment. We also found that the disappearance of some strains could be explained by cloning artifact due to polymerase chain reaction (PCR). Such artifact should be taken into account when analyzing this region by means of PCR.
丙型肝炎病毒基因组的高变区1引起了众多科学关注，因为据报道该区域较高的变异性是干扰素治疗反应不佳的一个预测因素。我们分析了两个疗程干扰素治疗前、治疗期间和治疗后的该区域氨基酸序列的连续变化。治疗前可见的一种主要毒株被另一种毒株所取代，后者在治疗前是相对较小的群体。后来在干扰素治疗期间观察到两个较小群体的短暂出现。我们还发现，某些毒株的消失可能是由于聚合酶链反应（PCR）导致的克隆假象。通过PCR分析该区域时应考虑到这种假象。
