Hrabák A, Idei M, Temesi A
Department of Biochemistry I., Semmelweis University Medical School, Budapest, Hungary.
Life Sci. 1994;55(10):797-805. doi: 10.1016/0024-3205(94)00563-x.
L-arginine, the precursor of nitric oxide(NO) is provided mainly by extracellular sources in casein-elicited murine and rat peritoneal macrophages. Free extracellular L-arginine(Arg), esters, peptides and proteins containing Arg are the best sources in accordance with the fact that proteolytic activity is high in peritoneal macrophages. The recycling of Arg from citrulline(Cit) was observed but at a low rate. This situation is different from that in endothelial cells where half of Arg is recycled from citrulline. No significant anaplerotic reaction from glutamic acid(Glu) can be demonstrated in peritoneal macrophages.
L-精氨酸是一氧化氮（NO）的前体，在酪蛋白诱导的小鼠和大鼠腹膜巨噬细胞中，它主要由细胞外来源提供。游离的细胞外L-精氨酸（Arg）、酯类、含Arg的肽和蛋白质是最佳来源，这与腹膜巨噬细胞中蛋白水解活性高这一事实相符。观察到精氨酸从瓜氨酸（Cit）的再循环，但速率较低。这种情况与内皮细胞不同，在内皮细胞中，一半的精氨酸是从瓜氨酸再循环而来的。在腹膜巨噬细胞中，无法证明谷氨酸（Glu）有明显的回补反应。
