Tates A D, van Dam F J, de Zwart F A, van Teylingen C M, Natarajan A T
MGC-Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, State University of Leiden, The Netherlands.
Mutat Res. 1994 Sep 1;309(2):299-306. doi: 10.1016/0027-5107(94)90106-6.
A cloning assay with high cloning efficiency has been developed to detect spontaneous and induced 6-thioguanine-resistant T-lymphocytes (HPRT mutants) from the spleen of adult mice. The mean cloning efficiency in untreated male mice of 20-22 weeks old was 34.5 +/- 11.2% (SD) and the corresponding mutant frequency 0.7 +/- 0.8 (SD) x 10(-6). The cloning efficiencies obtained in this study are substantially higher than those reported previously by other investigators. Using this assay, it could be demonstrated that inhalation exposure of mice to 200, 500 or 1300 ppm of 1,3-butadiene for 6 h/day on 5 consecutive days caused a statistically significant induction of 6-thioguanine-resistant mutations in T-lymphocytes from spleens of adult mice exposed to 1300 ppm. The exposure to 1300 ppm resulted in a three-fold increase of the spontaneous mutant frequency. The mutant frequency after exposure to 500 ppm was higher than the control but the increase was not significant.
已开发出一种具有高克隆效率的克隆测定法，用于检测成年小鼠脾脏中自发产生的和经诱导产生的对6-硫代鸟嘌呤具有抗性的T淋巴细胞（次黄嘌呤磷酸核糖转移酶突变体）。20 - 22周龄未处理雄性小鼠的平均克隆效率为34.5 +/- 11.2%（标准差），相应的突变频率为0.7 +/- 0.8（标准差）x 10(-6)。本研究中获得的克隆效率显著高于其他研究者先前报道的效率。使用该测定法可以证明，成年小鼠连续5天每天吸入暴露于200、500或1300 ppm的1,3 - 丁二烯，每天6小时，暴露于1300 ppm的成年小鼠脾脏T淋巴细胞中，6-硫代鸟嘌呤抗性突变出现了具有统计学意义的诱导增加。暴露于1300 ppm导致自发突变频率增加了三倍。暴露于500 ppm后的突变频率高于对照组，但增加不显著。
