Mirza M S, Hameed S, Akkermans A D
Department of Microbiology, Wageningen Agricultural University, The Netherlands.
Appl Environ Microbiol. 1994 Jul;60(7):2371-6. doi: 10.1128/aem.60.7.2371-2376.1994.
The presence of Frankia strains in soil samples collected from northern areas of Pakistan was detected by inoculating Coriaria nepalensis and Datisca cannabina plants. The abundance of compatible Frankia strains in some areas was indicated by profuse nodulation of the host plants, whereas soil samples from other localities failed to result in nodulation. An oligonucleotide probe (COR/DAT) directed against the 16S rRNA gene of the endophytes of Coriaria and Datisca spp. that did not cross-react with the RNA gene of Frankia strains isolated from other hosts was developed. Genetic diversity among Frankia strains nodulating D. cannabina was determined by sequence analysis of the partial 16S rRNA gene amplified from nodules induced by soil samples from different localities by PCR. Four types of Frankia sequences and one non-Frankia sequence were detected by hybridization with a Frankia genus probe and the COR/DAT probe as well as by sequence analysis of the cloned PCR products.
通过接种尼泊尔马桑和大麻叶痹木植物，检测了从巴基斯坦北部地区采集的土壤样品中是否存在弗兰克氏菌菌株。一些地区宿主植物大量结瘤表明存在大量兼容的弗兰克氏菌菌株，而其他地区的土壤样品未能导致结瘤。针对尼泊尔马桑和大麻叶痹木内生菌16S rRNA基因开发了一种寡核苷酸探针（COR/DAT），该探针与从其他宿主分离的弗兰克氏菌菌株的RNA基因无交叉反应。通过对不同地区土壤样品诱导的根瘤中扩增的部分16S rRNA基因进行序列分析，确定了大麻叶痹木根瘤弗兰克氏菌菌株之间的遗传多样性。通过与弗兰克氏菌属探针和COR/DAT探针杂交以及对克隆的PCR产物进行序列分析，检测到四种类型的弗兰克氏菌序列和一种非弗兰克氏菌序列。
