Wolters D J, Van Dijk C, Zoetendal E G, Akkermans A D
Department of Plant Microorganism Interactions, Netherlands Institute of Ecology, Heteren, The Netherlands.
Mol Ecol. 1997 Oct;6(10):971-81. doi: 10.1046/j.1365-294x.1997.00265.x.
Ineffective Frankia endophytes were retrieved from various wet soils by using Alnus glutinosa clones as trapping plants. No pure cultures could be isolated from these ineffective nodules. Therefore, the phylogenetic position of these endophytes was determined by sequence analysis of cloned PCR products of bacterial 16S rDNA, derived from nodules. The results showed that all nodule endophytes belong to a hitherto undescribed cluster of the Frankia phylogenetic tree. The position of these uncultured ineffective Frankia nodule endophytes is different from that of the ineffective Frankia isolates derived from A. glutinosa nodules, even when originating from the same geographical location. This suggests a bias in current isolation techniques.
通过使用欧洲桤木克隆作为诱捕植物，从各种湿润土壤中获取了无效的弗兰克氏内生菌。无法从这些无效根瘤中分离出纯培养物。因此，通过对来自根瘤的细菌16S rDNA克隆PCR产物进行序列分析，确定了这些内生菌的系统发育位置。结果表明，所有根瘤内生菌都属于弗兰克氏系统发育树中一个迄今未描述的簇。这些未培养的无效弗兰克氏根瘤内生菌的位置与源自欧洲桤木根瘤的无效弗兰克氏分离株不同，即使它们来自相同的地理位置。这表明当前的分离技术存在偏差。
