大肠杆菌O3及肠集聚性大肠杆菌菌株17-2的O抗原多糖的结构研究

Structural studies of the O-antigenic polysaccharides of Escherichia coli O3 and the enteroaggregative Escherichia coli strain 17-2.

作者信息

Medina E C, Widmalm G, Weintraub A, Vial P A, Levine M M, Lindberg A A

机构信息

Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Sweden.

出版信息

Eur J Biochem. 1994 Aug 15;224(1):191-6. doi: 10.1111/j.1432-1033.1994.tb20011.x.

DOI:10.1111/j.1432-1033.1994.tb20011.x

PMID:7521299

Abstract

The polysaccharide part of the lipopolysaccharide isolated from an enteroaggregative Escherichia coli isolated from a young child with diarrhoea in Santiago, Chile (strain 17-2), has been investigated. Sugar and methylation analyses of native and partially degraded polysaccharide together with 1H-NMR and 13C-NMR spectroscopies revealed that the polysaccharide is composed of pentasaccharide repeating units. The structure of the repeating unit of E. coli strain 17-2 O-polysaccharide is: [formula: see text] The structure of the O-polysaccharide from E. coli O3 was shown to be identical to that of E. coli strain 17-2 by sugar and methylation analyses and by 1H-NMR and 13C-NMR spectroscopies.

摘要

对从智利圣地亚哥一名患腹泻幼儿分离出的肠聚集性大肠杆菌（菌株17 - 2）中分离得到的脂多糖的多糖部分进行了研究。对天然及部分降解的多糖进行糖和甲基化分析，并结合1H - NMR和13C - NMR光谱分析表明，该多糖由五糖重复单元组成。大肠杆菌菌株17 - 2 O - 多糖重复单元的结构为：[化学式：见正文] 通过糖和甲基化分析以及1H - NMR和13C - NMR光谱分析表明，大肠杆菌O3的O - 多糖结构与大肠杆菌菌株17 - 2的相同。