产肠毒素大肠杆菌（ETEC）O101 O抗原多糖的结构测定

Structure determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli (ETEC) O101.

作者信息

Staaf M, Urbina F, Weintraub A, Widmalm G

机构信息

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

出版信息

Carbohydr Res. 1997 Jan 17;297(3):297-9. doi: 10.1016/s0008-6215(96)00274-1.

DOI:10.1016/s0008-6215(96)00274-1

PMID:9060191

Abstract

The O-antigenic polysaccharide of the lipopolysaccharide from the enterotoxigenic Escherichia coli O101 has been investigated. The composition and sequence of the repeating units was established by sugar and methylation analysis together with 1H and 13C NMR spectroscopy. The sequence was corroborated using the computer program CASPER. The structure of the repeating unit of the polysaccharide from E. coli O101 is as follows: -->6)-alpha-D-GlcpNAc-1-->4-alpha-D-GalpNAc-(1-->.

摘要

对产肠毒素大肠杆菌O101脂多糖的O抗原多糖进行了研究。通过糖和甲基化分析以及1H和13C核磁共振光谱确定了重复单元的组成和序列。使用计算机程序CASPER证实了该序列。大肠杆菌O101多糖重复单元的结构如下：-->6)-α-D-氨基葡萄糖-1-->4-α-D-氨基半乳糖-(1-->。