Murakami S, Takahashi Y, Yoshida S, Fuke I, Ohmae K, Mori C, Takagi M, Takamizawa A, Okayama H
Kanonji Institute, Research Foundation for Microbial Diseases, Osaka University, Kagawa, Japan.
J Med Virol. 1994 Jun;43(2):175-81. doi: 10.1002/jmv.1890430215.
A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested polymerase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription-polymerase chain reaction (ORTPCR) detects as few as 3-5 virions in 0.1 ml of whole blood specimens. The effectiveness of this assay system is confirmed by diagnosis of human hepatitis C viral (HCV) infection.
开发了一种通过逆转录结合巢式聚合酶链反应对血液标本中的病毒RNA进行高灵敏度检测的方案。以日本脑炎病毒（JEV）为模型，优化的逆转录-聚合酶链反应（ORTPCR）可在0.1 ml全血标本中检测到低至3-5个病毒粒子。该检测系统的有效性通过对人类丙型肝炎病毒（HCV）感染的诊断得到证实。
