通过聚合酶链反应（PCR）增强丙型肝炎病毒（HCV）cDNA的扩增：检测存档血清中的HCV RNA
Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: detection of HCV RNA in archival sera.
作者信息
Beardsley A M, Gowans E J, Burrell C J, Marmion B P
机构信息
Infectious Diseases Laboratory, Institute of Medical and Veterinary Science, University of Adelaide, Australia.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1581-2. doi: 10.1128/jcm.34.6.1581-1582.1996.
Abstract
A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome.
摘要
一种能成功从保存不佳的存档血清中扩增丙型肝炎病毒RNA的逆转录聚合酶链反应（RT-PCR）检测方法得到了优化。使用莫洛尼鼠白血病病毒RNase H-逆转录酶，并通过对病毒基因组5'非翻译区的一个短（112碱基对）片段进行一轮PCR扩增，实现了最大灵敏度。
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