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[利用槲寄生(欧洲槲寄生)凝集素的铁蛋白缀合物进行细胞表面受体的电子显微镜定位]

[Use of ferritin conjugates of a lectin from mistletoe (Viscum album L.) for the electron microscopic localization of cell surface receptors].

作者信息

Wagner M, Wagner B, Franz H, Ziska P

出版信息

Acta Biol Med Ger. 1978;37(10):1537-42.

PMID:752206
Abstract

Ferritin conjugates of a lectin from mistletoe (Viscum album L.) were used for the electron-microscopic demonstration of carbohydrate receptors on the cell surface of human erythrocytes and murine tumor cells. Human A1 erythrocytes showed only a slight focal binding of ferritin. Cells of the mouse ascites tumor strain L 1210 were labelled very tightly on their surface and incorporate the ferritin by pinocytosis. Furthermore they showed cytotoxic changes in their ultrastructure. In the presence of galactose the labelling on the surface, the incorporation of the conjugate within the cell as well as the cytotoxicity were inhibited.

摘要

来自槲寄生(欧洲槲寄生)的一种凝集素的铁蛋白缀合物被用于在电子显微镜下展示人红细胞和鼠肿瘤细胞表面的碳水化合物受体。人A1红细胞仅显示出铁蛋白的轻微局灶性结合。小鼠腹水肿瘤株L 1210的细胞表面被紧密标记,并通过胞饮作用摄取铁蛋白。此外,它们在超微结构上显示出细胞毒性变化。在半乳糖存在的情况下,表面标记、缀合物在细胞内的摄取以及细胞毒性均受到抑制。

相似文献

1
[Use of ferritin conjugates of a lectin from mistletoe (Viscum album L.) for the electron microscopic localization of cell surface receptors].[利用槲寄生(欧洲槲寄生)凝集素的铁蛋白缀合物进行细胞表面受体的电子显微镜定位]
Acta Biol Med Ger. 1978;37(10):1537-42.
2
Influence of carbohydrates on the cytotoxicity of an aqueous mistletoe drug and of purified mistletoe lectins tested on human T-leukemia cells.碳水化合物对在人T白血病细胞上测试的槲寄生水剂药物和纯化的槲寄生凝集素细胞毒性的影响。
Arzneimittelforschung. 1989 Jun;39(6):647-51.
3
[Isolation and characterization of mistletoe extracts (Viscum album L.). II. Effect of agglutinating and cytotoxic fractions on mouse ascites tumor cells].[槲寄生提取物(欧洲槲寄生)的分离与特性鉴定。II. 凝集和细胞毒性组分对小鼠腹水肿瘤细胞的作用]
Acta Biol Med Ger. 1977;36(1):119-25.
4
Isolation and characterization of mistletoe extracts (Viscum album L.). I. Affinity chromatography of mistletoe extracts on immobilized plasma proteins.槲寄生提取物(欧洲槲寄生)的分离与特性研究。I. 槲寄生提取物在固定化血浆蛋白上的亲和色谱分析。
Acta Biol Med Ger. 1977;36(1):113-7.
5
Chemical modification studies on the D-galactopyranosyl binding lectin from the mistletoe Viscum album L.对欧洲槲寄生(Viscum album L.)中D-吡喃半乳糖结合凝集素的化学修饰研究
Acta Biol Med Ger. 1979;38(9):1361-3.
6
Electron microscopic visualization by the unlabelled lectin/antiferritin-antibody/ferritin method of lectin receptors on cell surfaces.
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7
[Allergy and lectins: action between IgE-mediated histamine release and glycoproteins from Viscum album L. (mistletoe)].[过敏与凝集素:IgE介导的组胺释放与欧洲槲寄生(槲寄生)糖蛋白之间的相互作用]
Acta Biol Med Ger. 1978;37(10):1623-8.
8
Further refinement of the description of the ligand-binding characteristics for the galactoside-binding mistletoe lectin, a plant agglutinin with immunomodulatory potency.对半乳糖苷结合型槲寄生凝集素(一种具有免疫调节活性的植物凝集素)的配体结合特性描述的进一步完善。
J Mol Recognit. 1997 May-Jun;10(3):139-47. doi: 10.1002/(SICI)1099-1352(199705/06)10:3<139::AID-JMR358>3.0.CO;2-R.
9
Isolation and properties of three lectins from mistletoe (Viscum album L.).三种来自槲寄生(欧洲槲寄生)的凝集素的分离与特性
Biochem J. 1981 May 1;195(2):481-4. doi: 10.1042/bj1950481.
10
[The agglutination of human erythrocytes and mouse ascites tumor cells by extracts from mistletoe (Viscum album L.)].
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