Luther P, Franz H, Haustein B, Bergmann K C
Acta Biol Med Ger. 1977;36(1):119-25.
Lectin from mistletoe (Viscum album L.) was studied for its relations with the toxins from Viscum album, ascites tumor cells of mouse, and human immunoglobulins. Using affinity chromatography on glutaraldehyde-crosslinked IgG (human) from viscum crude extract, a fraction was isolated which exhibited full agglutination capacity and high toxicity. The supernatant showed no agglutination capacity but a strong toxic effect on mouse ascites tumor cells. This toxic effect could not be influenced by further additions of insolubilized IgG. Chromatography on DEAE cellulose also gave agglutinating fractions with toxic effects and a non-agglutinating toxic portion. Column chromatography on Sephadex G 75 allowed separation of toxic from agglutinating components. The molecular weight of the toxin remaining after lectin removal was above 10,000. Lectin was found to bind more readily to mouse ascites tumor cells than to erythrocytes.
对槲寄生（欧洲槲寄生）凝集素与欧洲槲寄生毒素、小鼠腹水肿瘤细胞及人免疫球蛋白之间的关系进行了研究。利用亲和色谱法，从槲寄生粗提物中对戊二醛交联的人IgG进行分离，得到了一个具有完全凝集能力和高毒性的组分。上清液无凝集能力，但对小鼠腹水肿瘤细胞有强烈毒性作用。进一步添加不溶性IgG并不能影响这种毒性作用。在DEAE纤维素上进行色谱分离也得到了具有毒性作用的凝集组分和无凝集作用的毒性部分。在Sephadex G 75上进行柱色谱分离可将毒性成分与凝集成分分开。去除凝集素后剩余毒素的分子量大于10000。发现凝集素与小鼠腹水肿瘤细胞的结合比与红细胞的结合更容易。
