Lynch N R, Thomas W R, Chua Y, García N, Di Prisco M C, López R
Institute of Biomedicine, Central University of Venezuela, Caracas.
Int Arch Allergy Immunol. 1994 Sep;105(1):70-4. doi: 10.1159/000236805.
The cDNA coding for one of the major allergens of Dermatophagoides pteronyssinus, Der p II, has been cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The recombinant material (r Der p II) was tested in the skin of house-dust allergic patients, and was found to be almost as active as the purified native allergen (n Der p II) in stimulating immediate hypersensitivity reactions. The positivity to r Der p II in these patients, who were selected for their skin-test reactivity to house-dust extract (HDE) and whole mite extract (WME), was over 60%, and 80% of their sera contained detectable IgE antibody against the recombinant allergen. The level of specific IgE antibody against r Der p II was a mean of 40% of that against WME in the sera of patients who were skin test positive to both allergen preparations. In contrast to the house-dust allergic patients, none of a group of allergic patients whose clinical history did not implicate house dust, and who were skin test negative to HDE or WME, were positive to r Der p II. Of interest was the observation that only 37% of a group of subjects who were skin test positive to HDE and WME, but who reported no clinical history of allergic disease upon natural exposure to house dust, reacted to r Der p II. These results demonstrate the biological activity of r Der p II, and indicate, in persons skin test positive to house-dust mite extract, an association between reactivity to Der p II and the manifestation of clinical symptoms.
编码屋尘螨主要变应原之一Der p II的cDNA已被克隆，并在大肠杆菌中作为与谷胱甘肽S-转移酶的融合蛋白表达。重组物质（r Der p II）在屋尘过敏患者的皮肤中进行了测试，发现在刺激速发型超敏反应方面，其活性几乎与纯化的天然变应原（n Der p II）相同。这些因对屋尘提取物（HDE）和全螨提取物（WME）皮肤试验呈阳性反应而被挑选出的患者，对r Der p II的阳性率超过60%，且其血清中有80%含有可检测到的针对重组变应原的IgE抗体。在对两种变应原制剂皮肤试验均呈阳性的患者血清中，针对r Der p II的特异性IgE抗体水平平均为针对WME的特异性IgE抗体水平的40%。与屋尘过敏患者不同，一组临床病史与屋尘无关且对HDE或WME皮肤试验呈阴性的过敏患者，对r Der p II均无阳性反应。有趣的是，一组对HDE和WME皮肤试验呈阳性但报告在自然接触屋尘时无过敏性疾病临床病史的受试者中，只有37%对r Der p II有反应。这些结果证明了r Der p II的生物活性，并表明在对屋尘螨提取物皮肤试验呈阳性的人中，对Der p II的反应性与临床症状的表现之间存在关联。
