Levine S, Saltzman A
Nathan S. Kline Institute for Psychiatric Research, Orangeburg, NY 10962.
Int Arch Allergy Immunol. 1994 Sep;105(1):91-5. doi: 10.1159/000236808.
Rats were sensitized to chicken ovalbumin or human gamma-globulin by inoculation without adjuvants into the peritoneal cavity in the healing phase of a chemical peritonitis. This phase is associated with striking enhancement of lymphatic absorption. Small doses of antigen sensitized the rats for subsequent induction of anaphylaxis, but large doses were almost completely ineffective (inverse dose-response relation). When certain adjuvants were added to the antigen, both high and low doses of antigen were effective sensitizers for anaphylaxis. Neither high nor low doses of antigen sensitized if injected without adjuvants into the unprepared peritoneal cavity or by any other route. The effects of sensitization with low or high doses of antigen and the results of inoculation by effective and ineffective routes were interpreted in terms of the balance between absorption into the lymphatics and into the systemic blood circulation. Supplemental antigen inoculated into the systemic circulation was able to tip the balance against sensitization even when sensitization was done with potent adjuvants and by a favorable route. Splenectomy had little or no effect on suppression by supplemental antigen.
在化学性腹膜炎的愈合期，不给佐剂，将大鼠腹腔接种鸡卵白蛋白或人γ-球蛋白使其致敏。此阶段伴有淋巴吸收显著增强。小剂量抗原可使大鼠致敏，随后引发过敏反应，但大剂量几乎完全无效（剂量反应呈反比关系）。当向抗原中添加某些佐剂时，高剂量和低剂量抗原均为有效的过敏反应致敏剂。若不添加佐剂，将抗原注入未预处理的腹腔或通过任何其他途径注射，无论高剂量还是低剂量抗原均不能使大鼠致敏。根据淋巴吸收与体循环吸收之间的平衡来解释低剂量或高剂量抗原致敏的效果以及有效和无效接种途径的结果。即使致敏是通过有效的佐剂和有利的途径进行的，注入体循环的补充抗原也能够打破平衡，抑制致敏。脾切除术对补充抗原的抑制作用几乎没有影响或没有影响。
