Belaya Y A, Kostenko L S, Gekker V D
J Hyg Epidemiol Microbiol Immunol. 1977;21(3):285-94.
Using the method of immunoelectrophoresis in agar, we established the presence of 12 soluble antigens in Sh. dysenteriae (Shiga). We differentiated among them 3 specific antigens situated in the cathodic region. One of them is the somatic O antigen while the other two are thermolabile surface K antigens of these bacteria. K antigens of Grigoryev-Shiga bacteria differ from the earlier described K antigens of the Newcastle and Boyd Shigellae in their positive electric charge, in greater lability in relation to temperature and chemical effects and evidently also in their chemical character. The remaining protein antigens are common for either the genus of Shigella or for the family of enterobacteria. The exotoxin preparation isolated from the R strain of Sh. dysenteriae (Voile 30) by precipitation with trichloroacetic acid is represented in the immunophoreogram by five precipitation lines. Disc electrophoresis in polyacrylamide gel made possible the investigation of the composition of the exotoxin and isolation of the two fractions responsible for its toxic effect. The method of preparative electrophoresis in polyacrylamide gel can evidently be used for obtaining purified Sh. dysenteriae exotoxin preparations.
我们采用琼脂免疫电泳法，确定了痢疾志贺菌（志贺氏菌）中存在12种可溶性抗原。我们区分出其中3种位于阴极区的特异性抗原。其中一种是菌体O抗原，另外两种是这些细菌的不耐热表面K抗原。格里戈里耶夫-志贺菌的K抗原与先前描述的纽卡斯尔志贺菌和博伊德志贺菌的K抗原不同，其带正电荷，对温度和化学作用的稳定性更高，其化学性质也明显不同。其余的蛋白质抗原是志贺菌属或肠杆菌科共有的。通过用三氯乙酸沉淀从痢疾志贺菌R菌株（沃伊勒30）中分离出的外毒素制剂，在免疫电泳图谱中表现为五条沉淀线。聚丙烯酰胺凝胶圆盘电泳使研究外毒素的组成以及分离出对其毒性作用负责的两个组分成为可能。聚丙烯酰胺凝胶制备电泳法显然可用于获得纯化的痢疾志贺菌外毒素制剂。
