Sarker A B, Koirala T R, Nose S, Jeon H J, Hoshida Y
Department of Pathology, Okayama University Medical School, Japan.
Indian J Pathol Microbiol. 1994 Jan;37(1):45-51.
Immunoreactivity with monoclonal antibodies against epithelial membrane antigen (EMA), vimentin, squamous epithelium-specific keratin, nonsquamous epithelium-specific keratin, and polyclonal antibodies epithelial cells of 55 cervical smears using the avidin-biotin-peroxidase complex and indirect immunoperoxidase methods to detect antigens. Most of the abnormal squamous cells with few normal cells were reactive for EMA but the intensity of the reaction was variable in both cases. There was no correlation in the reactivity between normal and abnormal cells with different cytokeratins varying in their molecular weight. Vimentin was also reactive with both cells. The results of this experiment suggest that antibodies used, appear to be of limited usefulness in the diagnosis of cervical smears.
采用抗生物素蛋白-生物素-过氧化物酶复合物法和间接免疫过氧化物酶法，使用针对上皮膜抗原（EMA）、波形蛋白、鳞状上皮特异性角蛋白、非鳞状上皮特异性角蛋白的单克隆抗体以及多克隆抗体，检测55份宫颈涂片上皮细胞中的抗原。大多数异常鳞状细胞（正常细胞较少）对EMA呈反应性，但两种情况下反应强度均有变化。不同分子量的细胞角蛋白在正常细胞和异常细胞之间的反应性不存在相关性。波形蛋白对两种细胞也呈反应性。本实验结果表明，所使用的抗体在宫颈涂片诊断中似乎用处有限。
