Moncrieff D, Ormerod M G, Coleman D V
Acta Cytol. 1984 Jul-Aug;28(4):407-10.
The distribution of three tumor markers, epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA) and prekeratin (PK) was studied in exfoliated epithelial cells in cervical smears using an immunoalkaline phosphatase staining technique to demonstrate the antigens. EMA was expressed by abnormal cells in a consistent and reproducible fashion whereas the other two markers were variably expressed by both normal and abnormal cells. Our results suggest that immunocytochemical staining for EMA could be of value not only for the diagnosis of cervical intraepithelial neoplasia but also for the automated screening of cervical smears.
采用免疫碱性磷酸酶染色技术显示抗原，对宫颈涂片的脱落上皮细胞中三种肿瘤标志物，即上皮膜抗原（EMA）、癌胚抗原（CEA）和前角蛋白（PK）的分布进行了研究。EMA在异常细胞中以一致且可重复的方式表达，而其他两种标志物在正常细胞和异常细胞中均有不同程度的表达。我们的结果表明，EMA免疫细胞化学染色不仅对宫颈上皮内瘤变的诊断有价值，而且对宫颈涂片的自动筛查也有价值。
