大鼠虹膜中酪氨酸羟化酶、P物质和降钙素基因相关肽免疫反应性神经纤维的超微结构分析
Ultrastructural analysis of tyrosine hydroxylase-, substance P-, and calcitonin gene-related peptide-immunoreactive nerve fibers in the rat iris.
作者信息
Hirai R, Tamamaki N, Hukami K, Nojyo Y
机构信息
Department of Ophthalmology, Fukui Medical School, Japan.
出版信息
Ophthalmic Res. 1994;26(3):169-80. doi: 10.1159/000267409.
Different patterns of distribution of chemically different nerve fibers in the rat iris were studied by immunoelectron microscopy. The iris was immunostained either singly for tyrosine hydroxylase (TH), or doubly for substance P (SP) and calcitonin gene-related peptide (CGRP), or triply for TH, SP and CGRP. TH-positive fibers were distributed in the stroma near the iris dilator and within the iris sphincter. SP/CGRP-positive fibers were found mainly in the anterior half of the stroma and partly in the stroma close to the sphincter, but were hardly ever seen in the stroma near the dilator or within the sphincter. Fibers neither positive for TH, SP nor CGRP were observed in the stroma near the dilator and within the sphincter.
通过免疫电子显微镜研究了大鼠虹膜中化学性质不同的神经纤维的不同分布模式。虹膜分别用酪氨酸羟化酶（TH）进行单免疫染色，或用P物质（SP）和降钙素基因相关肽（CGRP）进行双免疫染色，或用TH、SP和CGRP进行三免疫染色。TH阳性纤维分布在虹膜开大肌附近的基质和虹膜括约肌内。SP/CGRP阳性纤维主要见于基质的前半部分，部分见于靠近括约肌的基质中，但在开大肌附近的基质或括约肌内几乎未见。在开大肌附近的基质和括约肌内未观察到TH、SP或CGRP均为阴性的纤维。
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