[大鼠虹膜神经支配的神经纤维分布及密度的免疫电子显微镜研究]

[An immunoelectron microscopic study of distribution and density of nerve fibers innervating the rat iris].

作者信息

Hirai R, Tamamaki N, Ishiguro H, Hukami K, Nojyo Y

机构信息

Department of Opthalmology, Fukui Medical School, Fukui-ken, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1994 Feb;98(2):130-6.

PMID:8109456

Abstract

We studied the sympathetic, parasympathetic and sensory nerve fibers in the rat iris using electron microscopic immunohistochemical techniques. Antibodies raised against tyrosine hydroxylase (TH), substance P (SP), and calcitonin gene-related peptide (CGRP) were used alone or combined together to characterize the nerve fibers. The ratios of the TH-positive, SP/CGRP-positive and TH/SP/CGRP-negative fibers were 18%, 37%, and 45% in the anterior half of the dilator stroma, 11%, 3%, and 86% in the posterior half of the dilator stroma, 45%, 26%, and 29% in the sphincter stroma, and 23%, 1%, and 76% in the sphincter muscle layer. These results confirm that the sympathetic and parasympathetic fibers doubly innervate both rat iris sphincter and iris dilator muscles, whereas sensory nerve fibers innervate mainly the stroma.

摘要

我们使用电子显微镜免疫组织化学技术研究了大鼠虹膜中的交感神经、副交感神经和感觉神经纤维。分别使用针对酪氨酸羟化酶（TH）、P物质（SP）和降钙素基因相关肽（CGRP）产生的抗体，或联合使用这些抗体来鉴定神经纤维。在瞳孔开大肌基质前半部分，TH阳性、SP/CGRP阳性和TH/SP/CGRP阴性纤维的比例分别为18%、37%和45%；在瞳孔开大肌基质后半部分，上述比例分别为11%、3%和86%；在瞳孔括约肌基质中，上述比例分别为45%、26%和29%；在瞳孔括约肌肌层中，上述比例分别为23%、1%和76%。这些结果证实，交感神经纤维和副交感神经纤维双重支配大鼠虹膜括约肌和虹膜开大肌，而感觉神经纤维主要支配基质。