Beckers H J, Klooster J, Vrensen G F, Lamers W P
Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Ophthalmic Res. 1993;25(3):192-200. doi: 10.1159/000267291.
The distribution of substance P (SP) within the nerve fibers of the rat cornea and iris was studied using electron microscopic immunohistochemistry. Numerous SP-immunoreactive fibers were found throughout the corneal and iridal stroma. The most intense staining was found within varicosities containing numerous vesicles. Similar SP-immunoreactive varicosities were found in fibers in close proximity to the iris sphincter and dilator muscles. On account of previous ultrastructural work the SP-containing fibers and varicosities must be assumed to be of trigeminal origin. Additionally, the distribution of SP and calcitonin gene-related peptide (CGRP) in corneal and iridal fibers of rats is similar, suggesting that CGRP and SP are colocalized and may be coreleased from the same varicosities.
采用电子显微镜免疫组织化学方法研究了P物质（SP）在大鼠角膜和虹膜神经纤维中的分布。在整个角膜和虹膜基质中发现了大量SP免疫反应性纤维。在含有大量囊泡的膨体中发现了最强的染色。在靠近虹膜括约肌和开大肌的纤维中也发现了类似的SP免疫反应性膨体。根据先前的超微结构研究，必须假定含SP的纤维和膨体起源于三叉神经。此外，大鼠角膜和虹膜纤维中SP和降钙素基因相关肽（CGRP）的分布相似，表明CGRP和SP共定位，可能从同一膨体中共同释放。
