Menasce L P, Orphanos V, Santibanez-Koref M, Boyle J M, Harrison C J
Department of Histopathology, Christie Hospital, Manchester, UK.
Genes Chromosomes Cancer. 1994 Aug;10(4):286-8. doi: 10.1002/gcc.2870100411.
We have used fluorescence in situ hybridisation (FISH) with a series of yeast artificial chromosome (YAC) clones that map to the long arm of chromosome 6 (6q) to define the region(s) of deletion in seven cases of non-Hodgkin's lymphoma (NHL), in which a deletion of 6q had been detected by conventional cytogenetics. The FISH analysis detected two regions of deletion: (i) A proximal region flanked by M6P1 (6q14-15) and FYN (6q21), containing D6S246, which was missing in all seven cases. This locus was also found to be deleted in all six cases of acute lymphoblastic leukaemia (ALL) studied previously. (ii) A second region of 6q, which was distal to 6q23.1 (D6S238) and included ESR (6q25.1) and D6S281 (6q27), which was shown to be present in all our cases of ALL, was found to be deleted in 4 of the 7 cases of NHL. Our results support the suggestion that tumour suppressor genes, involved in the pathogenesis of lymphoid malignancies, may be present within these regions.
我们使用了一系列定位于6号染色体长臂（6q）的酵母人工染色体（YAC）克隆进行荧光原位杂交（FISH），以确定7例非霍奇金淋巴瘤（NHL）中的缺失区域，在这些病例中，通过传统细胞遗传学检测到6q存在缺失。FISH分析检测到两个缺失区域：（i）一个近端区域，两侧分别为M6P1（6q14 - 15）和FYN（6q21），包含D6S246，在所有7例病例中均缺失。该位点在先前研究的所有6例急性淋巴细胞白血病（ALL）中也被发现缺失。（ii）6q的第二个区域，位于6q23.1（D6S238）远端，包括ESR（6q25.1）和D6S281（6q27），在我们所有的ALL病例中均显示存在，但在7例NHL病例中的4例中被发现缺失。我们的结果支持这样的观点，即参与淋巴恶性肿瘤发病机制的肿瘤抑制基因可能存在于这些区域内。
