Randerson J, Cawkwell L, Jack A, Lewis F, Johnson P, Evans P, Barrans S, Morgan G J
Department of Clinical Sciences: Pathological Science, University of Leeds, UK.
Br J Cancer. 1996 Sep;74(6):942-6. doi: 10.1038/bjc.1996.461.
Twenty-four cases of histologically defined follicle centre cell (FCC) lymphoma have been examined for allele imbalance at 19 microsatellite loci spanning the length of chromosome 6, including six markers within the major histocompatibility complex (MHC), using fluorescent polymerase chain reaction (PCR) to amplify microsatellites. Nineteen cases were observed in which imbalance of one or more markers on chromosome 6 had occurred (79%). The frequency of allele imbalance was significantly higher on 6p than 6q, and two regions of deletions, 6p24-25 and 6p21.3-23, were identified in which the loci showed a significantly high allele imbalance frequency.
对24例经组织学确诊的滤泡中心细胞（FCC）淋巴瘤进行了检测，以研究跨越6号染色体全长的19个微卫星位点的等位基因失衡情况，其中包括主要组织相容性复合体（MHC）内的6个标记，采用荧光聚合酶链反应（PCR）扩增微卫星。观察到19例出现6号染色体上一个或多个标记失衡的情况（79%）。6p上等位基因失衡的频率显著高于6q，并且确定了两个缺失区域，即6p24 - 25和6p21.3 - 23，其中的位点显示出显著高的等位基因失衡频率。
