人脑中谷氨酸转运体cDNA的克隆及特性分析 以及 来自人胰腺的谷氨酸转运体cDNA的克隆及特性分析 （原标题表述易引起歧义，从字面上看可理解为两个研究：人脑中谷氨酸转运体cDNA的克隆及特性分析，以及人胰腺中谷氨酸转运体cDNA的克隆及特性分析，但从单个标题来说不太明确具体是否是这两个研究内容，这里按照字面意思翻译供你参考）
Cloning and characterization of a glutamate transporter cDNA from human brain and pancreas.
作者信息
Manfras B J, Rudert W A, Trucco M, Boehm B O
机构信息
Department of Internal Medicine I, School of Medicine, University of Ulm, Germany.
出版信息
Biochim Biophys Acta. 1994 Oct 12;1195(1):185-8. doi: 10.1016/0005-2736(94)90026-4.
L-Glutamate is the major excitatory neurotransmitter in the brain. Sufficient removal from the synaptic cleft after neurotransmission by the L-glutamate transport system is essential to prevent excitotoxicity and neurotoxicity. We isolated mRNA from human brain and pancreatic islet cells and screened for sequences of high homology to a previously characterized rat brain glutamate transporter. An isolated sequence (GLTR) shows a 87.5% and a 92.5% sequence similarity at the nucleotide and amino acid level, respectively, with a rat brain specific L-glutamate transporter but only a 65% homology to the recently cloned human glutamate/aspartate transporter. The human mRNA is differentially expressed in brain and to a lesser degree in pancreas and in fetal liver. The gene encoding for the newly identified cDNA is located on chromosome 5.
L-谷氨酸是大脑中的主要兴奋性神经递质。神经传递后，L-谷氨酸转运系统从突触间隙充分清除谷氨酸对于预防兴奋性毒性和神经毒性至关重要。我们从人脑中分离出mRNA，并筛选与先前鉴定的大鼠脑谷氨酸转运体具有高度同源性的序列。一个分离的序列（GLTR）在核苷酸和氨基酸水平上分别与大鼠脑特异性L-谷氨酸转运体显示出87.5%和92.5%的序列相似性，但与最近克隆的人谷氨酸/天冬氨酸转运体只有65%的同源性。人mRNA在脑中差异表达，在胰腺和胎儿肝脏中表达程度较低。编码新鉴定cDNA的基因位于5号染色体上。
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