Okamura H, Miyagawa A, Takagi H, Esumi H, Yanaihara N, Ibata Y
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Neuroreport. 1994 Jun 2;5(10):1177-80. doi: 10.1097/00001756-199406020-00004.
The possible co-existence of pituitary adenylate cyclase-activating polypeptide (PACAP)-38 and nitric oxide synthase (NOS) in the rat hypothalamus was examined by a combination of PACAP-immunocytochemistry and NADPH-diaphorase histochemistry. Virtually all PACAP-38-immunoreactive neurones in the paraventricular and supraoptic nuclei exhibited NADPH-diaphorase activity. Since NADPH-diaphorase activity was identical to NOS-immunoreactivity in the magnocellular neurosecretory neurones, this finding indicates that the PACAP neurones synthesize NO.
