Miyagawa A, Okamura H, Ibata Y
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Neurosci Lett. 1994 Apr 25;171(1-2):13-6. doi: 10.1016/0304-3940(94)90592-4.
Nitric oxide (NO), which was firstly identified as an endothelium-derived relaxing factor, has recently been demonstrated to be a neurotransmitter in the central and peripheral nervous systems. In the hypothalamus, abundant nitric oxide synthase (NOS) immunoreactivity and its histochemical marker, NADPH-diaphorase activity, have been demonstrated in the hypothalamo-neurohypophyseal system. In the present study, we examined whether NOS is coexpressed with posterior pituitary hormones in the rat hypothalamus by combination of oxytocin and vasopressin immunofluorescence and NADPH-diaphorase histochemistry. Most oxytocin-immunoreactive neurons in the paraventricular and supraoptic nuclei expressed NADPH-diaphorase activity, but virtually no vasopressin-immunoreactive neurons contained NADPH-diaphorase activity. This suggests that oxytocin neurons are the main source of NO production in the hypothalamic-pituitary system.
一氧化氮（NO）最初被鉴定为一种内皮衍生舒张因子，最近已被证明是中枢和外周神经系统中的一种神经递质。在下丘脑中，下丘脑 - 神经垂体系统中已证实存在丰富的一氧化氮合酶（NOS）免疫反应性及其组织化学标记物，即NADPH - 黄递酶活性。在本研究中，我们通过催产素和血管加压素免疫荧光与NADPH - 黄递酶组织化学相结合的方法，研究了大鼠下丘脑中NOS是否与垂体后叶激素共表达。室旁核和视上核中的大多数催产素免疫反应性神经元表达NADPH - 黄递酶活性，但几乎没有血管加压素免疫反应性神经元含有NADPH - 黄递酶活性。这表明催产素神经元是下丘脑 - 垂体系统中NO产生的主要来源。
