Leitch G J, He Q
Department of Physiology, Morehouse School of Medicine, Atlanta, Georgia 30310-1495.
Infect Immun. 1994 Nov;62(11):5173-6. doi: 10.1128/iai.62.11.5173-5176.1994.
Dietary L-arginine (4%) significantly reduced fecal oocyst shedding in athymic nude mice chronically infected with Cryptosporidium parvum. This effect appeared to be due to an increase in host nitric oxide (NO) production as it was not observed in arginine-supplemented animals administered the NO synthase inhibitor, N-nitro-L-arginine methyl ester. N-Nitro-L-arginine methyl ester alone significantly increased fecal oocyst shedding in chronically infected animals. In in vitro assays, oocyst excystation and sporozoite viability were significantly reduced by the NO donors sodium nitroprusside and S-nitroso-L-acetyl penicillamine in a concentration-dependent manner. These data suggest that arginine-derived NO may reduce the parasite load in experimental cryptosporidiosis.
饮食中的L-精氨酸（4%）可显著减少长期感染微小隐孢子虫的无胸腺裸鼠粪便中卵囊的排出量。这种作用似乎是由于宿主一氧化氮（NO）生成增加所致，因为在补充精氨酸并给予NO合酶抑制剂N-硝基-L-精氨酸甲酯的动物中未观察到这种作用。单独使用N-硝基-L-精氨酸甲酯可显著增加慢性感染动物粪便中卵囊的排出量。在体外试验中，NO供体硝普钠和S-亚硝基-L-乙酰青霉胺以浓度依赖的方式显著降低了卵囊脱囊和子孢子活力。这些数据表明，精氨酸衍生的NO可能会降低实验性隐孢子虫病中的寄生虫负荷。
