Dahlqvist A, Neuhuber W L, Forsgren S
Department of Otorhinolaryngology, University of Umeå, Sweden.
J Comp Neurol. 1994 Jul 15;345(3):440-6. doi: 10.1002/cne.903450310.
Carotid body-like organs, paraganglia, frequently occur in the superior and recurrent laryngeal nerves. The paraganglia are supplied with a rich innervation of unknown origin. In the present study, the origin of the innervation of the paraganglia of the rat was studied with two different techniques. One approach was anterograde tracing of wheat-germ agglutinin-horseradish peroxidase after injection into the nodose and jugular ganglia of the vagus and the superior cervical ganglion. The other approach was immunohistochemical staining for neuropeptides after excision of the superior cervical ganglion, or vagotomy. Antisera against neuropeptide Y, vasoactive intestinal polypeptide, and calcitonin gene-related peptide were utilized. Both the tracing method and calcitonin gene-related peptide immunohistochemistry after vagotomy showed that the paraganglia receive sensory innervation from the vagal ganglia. No labeling was detected in the paraganglia after injection of wheat-germ agglutinin-horseradish peroxidase in the superior cervical ganglion. Excision of this ganglion did not lead to a decrease in the neuropeptide-Y innervation in the paraganglia, but most of this innervation in the surrounding blood vessels disappeared. The observations show that the superior cervical ganglion does not contribute to the innervation in the paraganglia and that the neuropeptide-Y innervation of the blood vessels originates from the superior cervical ganglion whereas that of the paraganglia has another origin, most likely local ganglionic cells. The results also suggest that the vasoactive intestinal polypeptide innervation in the paraganglia arises from local ganglionic cells. The two approaches complemented each other in mapping the afferent and efferent nerve supply of the paraganglia.
类颈动脉体副神经节常出现在喉上神经和喉返神经中。这些副神经节由来源不明的丰富神经支配。在本研究中，采用两种不同技术研究了大鼠副神经节神经支配的来源。一种方法是将小麦胚芽凝集素 - 辣根过氧化物酶注入迷走神经的结状神经节和颈静脉神经节以及颈上神经节后进行顺行追踪。另一种方法是在切除颈上神经节或切断迷走神经后对神经肽进行免疫组织化学染色。使用了抗神经肽Y、血管活性肠肽和降钙素基因相关肽的抗血清。追踪方法和切断迷走神经后降钙素基因相关肽免疫组织化学均显示副神经节接受来自迷走神经节的感觉神经支配。在颈上神经节注射小麦胚芽凝集素 - 辣根过氧化物酶后，副神经节未检测到标记。切除该神经节并未导致副神经节中神经肽Y神经支配减少，但周围血管中的大部分这种神经支配消失。这些观察结果表明，颈上神经节对副神经节的神经支配没有贡献，血管的神经肽Y神经支配起源于颈上神经节，而副神经节的神经肽Y神经支配有另一个来源，很可能是局部神经节细胞。结果还表明，副神经节中的血管活性肠肽神经支配起源于局部神经节细胞。这两种方法在绘制副神经节的传入和传出神经供应方面相互补充。
