Elsås T, Edvinsson L, Sundler F, Uddman R
Department of Ophthalmology, University of Trondheim, Norway.
Exp Eye Res. 1994 Jan;58(1):117-26. doi: 10.1006/exer.1994.1201.
The origin and neuropeptide content of nerve fibres in the rat conjunctiva were studied by retrograde tracing and denervations in combination with immunocytochemistry. Immunocytochemistry revealed nerve fibres containing neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI), helospectin, substance P (SP), and calcitonin gene-related peptide (CGRP) around blood vessels, smooth muscle bundles and glands. Removal of the sphenopalatine ganglion eliminated the majority of the VIP-, PHI- and helospectin-containing fibres. Sympathectomy eliminated the majority of the NPY-containing fibres in the conjunctiva. Removal of both the sphenopalatine ganglion and the superior cervical ganglion led to further reduction of the NPY fibres. Injection of the retrograde tracer True Blue into the conjunctiva resulted in the appearance of labelled cell bodies in the trigeminal ganglion, the sphenopalatine ganglion, the superior cervical ganglion, and the ciliary ganglion. Judging from the number of labelled nerve cell bodies, the superior cervical ganglion contributes most to the innervation. These results indicate that the majority of NPY-containing nerve fibres in the conjunctiva derives from the superior cervical ganglion (sympathetic nerve supply). Most of the VIP- and a minor population of the NPY-containing fibres in the conjunctiva originate in the sphenopalatine ganglion (parasympathetic nerve supply). A sparse amount of VIP- and NPY-containing fibres derives from the ciliary ganglion. Nerve fibres containing SP and/or CGRP emanate from the trigeminal ganglia (sensory nerve supply). Together the findings indicate that several ganglia project to the conjunctiva and that many neuropeptides may be involved in the control of conjunctival activity.
通过逆行追踪和去神经支配结合免疫细胞化学方法，研究了大鼠结膜神经纤维的起源和神经肽含量。免疫细胞化学显示，在血管、平滑肌束和腺体周围存在含有神经肽Y（NPY）、血管活性肠肽（VIP）、肽组氨酸异亮氨酸（PHI）、海洛视蛋白、P物质（SP）和降钙素基因相关肽（CGRP）的神经纤维。切除蝶腭神经节后，大部分含VIP、PHI和海洛视蛋白的纤维消失。交感神经切除后，结膜中大部分含NPY的纤维消失。同时切除蝶腭神经节和颈上神经节导致NPY纤维进一步减少。将逆行示踪剂真蓝注入结膜后，在三叉神经节、蝶腭神经节、颈上神经节和睫状神经节中出现了标记的细胞体。从标记神经细胞体的数量来看，颈上神经节对神经支配的贡献最大。这些结果表明，结膜中大部分含NPY的神经纤维来自颈上神经节（交感神经供应）。结膜中大部分含VIP的纤维和一小部分含NPY的纤维起源于蝶腭神经节（副交感神经供应）。少量含VIP和NPY的纤维来自睫状神经节。含有SP和/或CGRP的神经纤维来自三叉神经节（感觉神经供应）。这些发现共同表明，多个神经节投射到结膜，许多神经肽可能参与结膜活动的控制。
