Lidegran M, Domeij S, Dahlqvist A, Henriksson R, Franzén L, Gustafsson H, Forsgren S
Department of Otorhinolaryngology, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
Cell Tissue Res. 1995 Jan;279(1):55-63. doi: 10.1007/BF00300691.
The effects of radiotherapy on neuropeptide expression in the rat larynx were studied. Irradiation was given for five days, 6 or 8 Gray daily. Ten days after the end of irradiation, the larynx, the laryngeal nerves and different ganglia related to the larynx were dissected out from irradiated and control animals and processed for neuropeptide immunohistochemistry. There was an increased immunolabelling for two of the neuropeptides tested, substance P and enkephalin, in the innervation of the subglottic glands and in the acetylcholinesterase-positive ganglionic cells of the local ganglia. These cells were interpreted as representing postganglionic parasympathetic ganglionic cells. The changes seen in the subglottic glands were interpreted as most likely being related to the changing pattern of staining seen in the local ganglia. No changes in substance P- and enkephalin expression were observed in other laryngeal structures, the nodose ganglia, superior cervical ganglia or laryngeal nerve paraganglia. Thus, in certain respects neuropeptide expression in the larynx is modulated by radiotherapy. Since neuropeptides have both neurotransmitter and/or neuromodulator effects in airway tissue and since they show effects as growth factors, the occurrence of this plasticity in neuropeptide expression should be taken into consideration in future studies examining the effects of irradiation on normal/diseased airway tissues.
研究了放疗对大鼠喉部神经肽表达的影响。放疗持续五天，每天给予6或8格雷剂量。放疗结束十天后，从接受放疗的动物和对照动物中取出喉部、喉神经以及与喉部相关的不同神经节，进行神经肽免疫组织化学处理。在声门下腺的神经支配以及局部神经节中乙酰胆碱酯酶阳性的神经节细胞中，所检测的两种神经肽——P物质和脑啡肽的免疫标记有所增加。这些细胞被认为代表节后副交感神经节细胞。声门下腺中观察到的变化被解释为很可能与局部神经节中染色模式的改变有关。在其他喉部结构、结状神经节、颈上神经节或喉神经旁神经节中，未观察到P物质和脑啡肽表达的变化。因此，在某些方面，喉部的神经肽表达受放疗调节。由于神经肽在气道组织中兼具神经递质和/或神经调节剂的作用，且表现出作为生长因子的效应，在未来研究放疗对正常/患病气道组织的影响时，应考虑神经肽表达这种可塑性的存在。
