Bodenmüller H
Boehringer Mannheim GmbH, Research Center Tutzing, Germany.
Scand J Clin Lab Invest Suppl. 1995;221:60-6. doi: 10.3109/00365519509090566.
Soluble forms of keratins in human sera seem to be useful analytes for the monitoring of cancer patients. CYFRA 21-1 is a new test measuring keratin 19 in human blood. The test was developed as sandwich ELISA based on two monoclonal antibodies, both reacting with keratin 19. The epitopes recognised by the antibodies are located on the rod region of the molecule. In sera from malignant patients CYFRA 21-1 detects immunoreactive compounds which appear to be larger than keratin 19 itself, indicating the presence of oligomers in these sera. In a comparison study the reactivity of other keratin tests (TPA, TPS, TPAcyk) with the keratins 8, 18 and 19 were measured both in solution and on immunoblots. The reactivity pattern was found to be different what may explain the different diagnostic properties of the individual tests.
人血清中可溶性角蛋白形式似乎是监测癌症患者的有用分析物。CYFRA 21-1是一种检测人血液中角蛋白19的新测试。该测试是基于两种均与角蛋白19反应的单克隆抗体开发的夹心酶联免疫吸附测定。抗体识别的表位位于分子的杆状区域。在恶性患者的血清中，CYFRA 21-1检测到的免疫反应性化合物似乎比角蛋白19本身更大，表明这些血清中存在寡聚体。在一项比较研究中，在溶液中和免疫印迹上测量了其他角蛋白测试（TPA、TPS、TPAcyk）与角蛋白8、18和19的反应性。发现反应模式不同，这可能解释了各个测试不同的诊断特性。
